Aghil Sharifzadeh1, Ali Reza Khosravi2, Hojjatollah Shokri3, Paria Samadi Tari2. 1. Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Electronic address: asharifzadeh@ut.ac.ir. 2. Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 3. Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Abstract
BACKGROUND: Candida glabrata (C. glabrata) and C. krusei are now emerging as serious hospital acquired infections in immunocompromised patients. Menthol, a terpenic compound, has been reported to have antifungal activity. OBJECTIVES: The aim of this study was to investigate the effect of menthol in combination with itraconazole or nystatin against C. glabrata and C. krusei isolates. METHODS: The effects of menthol along with itraconazole and nystatin, were evaluated by the Clinical Laboratory Standards Institute (CLSI) M44-A and CLSI M27-A3 methods. The fractional inhibitory concentration index (FICI) was determined for menthol plus itraconazole and nystatin combinations using the checkerboard method. RESULTS: The mean of minimum inhibitory concentration (MIC) values of menthol, nystatin and itraconazole were 53.2, 2.30 and 1.50 μg/ml for C. glabrata isolates and 121, 1.08 and 0.38 μg/ml for C. krusei isolates, respectively. Menthol in combination with itraconazole or nystatin exhibited the synergistic effects against all species of Candida tested. FICI values for menthol plus itraconazole and nystatin combinations ranged from 0.250 to 0.561 and 0.139 to 0.623 for C. glabrata isolates, and 0.182 to 0.750 and 0.188 to 0.760 for C. krusei, respectively. CONCLUSIONS: These results support the potential use of menthol as an anticandidal agent, and it can be used complementarily with other conventional antifungal agents.
BACKGROUND:Candida glabrata (C. glabrata) and C. krusei are now emerging as serious hospital acquired infections in immunocompromised patients. Menthol, a terpenic compound, has been reported to have antifungal activity. OBJECTIVES: The aim of this study was to investigate the effect of menthol in combination with itraconazole or nystatin against C. glabrata and C. krusei isolates. METHODS: The effects of menthol along with itraconazole and nystatin, were evaluated by the Clinical Laboratory Standards Institute (CLSI) M44-A and CLSI M27-A3 methods. The fractional inhibitory concentration index (FICI) was determined for menthol plus itraconazole and nystatin combinations using the checkerboard method. RESULTS: The mean of minimum inhibitory concentration (MIC) values of menthol, nystatin and itraconazole were 53.2, 2.30 and 1.50 μg/ml for C. glabrata isolates and 121, 1.08 and 0.38 μg/ml for C. krusei isolates, respectively. Menthol in combination with itraconazole or nystatin exhibited the synergistic effects against all species of Candida tested. FICI values for menthol plus itraconazole and nystatin combinations ranged from 0.250 to 0.561 and 0.139 to 0.623 for C. glabrata isolates, and 0.182 to 0.750 and 0.188 to 0.760 for C. krusei, respectively. CONCLUSIONS: These results support the potential use of menthol as an anticandidal agent, and it can be used complementarily with other conventional antifungal agents.
Authors: Arif Khan; Mohd Azam; Masood Alam Khan; Khaled S Allemailem; Faris Alrumaihi; Ahmad Almatroudi; Fahad A Alhumaydhi; Hafiz Iqtidar Ahmad; Masih Uzzaman Khan Journal: Infect Drug Resist Date: 2021-04-21 Impact factor: 4.003
Authors: Bruna Maria Pereira da Costa Cordeiro; Nataly Diniz de Lima Santos; Magda Rhayanny Assunção Ferreira; Larissa Cardoso Corrêa de Araújo; Alexsander Rodrigues Carvalho Junior; Alan Diego da Conceição Santos; Ana Paula de Oliveira; Alexandre Gomes da Silva; Emerson Peter da Silva Falcão; Maria Tereza Dos Santos Correia; Jackson Roberto Guedes da Silva Almeida; Luís Cláudio Nascimento da Silva; Luiz Alberto Lira Soares; Thiago Henrique Napoleão; Márcia Vanusa da Silva; Patrícia Maria Guedes Paiva Journal: BMC Complement Altern Med Date: 2018-10-19 Impact factor: 3.659