Ziba Nariman-Saleh-Fam1, Milad Bastami2, Mohammad Hossein Somi3, Farkhondeh Behjati4, Yaser Mansoori1, Abdolreza Daraei5, Zahra Saadatian6, Lida Nariman-Saleh-Fam7, Habibollah Mahmoodzadeh8, Yashar Makhdoumi9, Fatemeh Varshoee Tabrizi9, Bahador Ebrahimi-Sharif10, Azam Hezarian11, Shahnaz Naghashi3, Mohammad Reza Abbaszadegan12, Javad Tavakkoly-Bazzaz1. 1. 1 Medical Genetics Department, School of Medicine, Tehran University of Medical Sciences , Tehran, Iran . 2. 2 Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz, Iran . 3. 3 Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences , Tabriz, Iran . 4. 4 Genetics Research Center, University of Social Welfare and Rehabilitation Sciences , Tehran, Iran . 5. 5 Genetics Department, Faculty of Medicine, Babol University of Medical Sciences , Babol, Iran . 6. 6 Medical Genetics Department, School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran . 7. 7 Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz, Iran . 8. 8 Cancer Institute, Imam Khomeini Hospital, Tehran University of Medical Sciences , Tehran, Iran . 9. 9 Reza Radiation Oncology Center , Mashhad, Iran . 10. 10 Department of Genetics and Biotechnology, Varamin-Pishva Branch, Islamic Azad University , Varamin, Iran . 11. 11 Medical Laboratory, Modarres Hospital, Shahid Beheshti University of Medical Sciences , Tehran, Iran . 12. 12 Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences , Mashhad, Iran .
Abstract
AIMS: Iran is located in the Asian esophageal cancer belt. It is a high-risk region for esophageal squamous cell carcinoma (ESCC). The extent to which genetic components, especially variants within miRNAs or their binding sites, contribute to risk of ESCC in the region is not yet fully understood. Herein, tests were done on an Iranian cohort to evaluate the association of miRNA-related polymorphisms in miR-423 (rs6505162) and peroxisomal biogenesis factor 6 (PEX6) (rs1129186 within a miR-149-5p-binding site) with the risk of ESCC risk. METHODS: This study recruited 200 ESCC patients and 300 healthy individuals. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism method. Target genes and biological processes that are regulated by miR-423 and may be affected by a change in miR-423 expression were identified by in silico analysis. RESULTS: Logistic regression analyses revealed an association between rs6505162 and ESCC, assuming codominant (AA vs. CC, odds ratios, OR [95% confidence interval, CI]: 0.32 [0.15-0.69], p-value: 0.0076), recessive (AA vs. CC+CA, OR [95% CI]: 0.35 [0.16-0.73], p-value: 0.0027), and log-additive models (OR [95% CI]: 0.69 [0.52-0.91], p-value: 0.0084). No significant association was observed for PEX6 rs1129186. In silico analyses revealed several genes and biological processes that are regulated by miR-423 in ESCC. CONCLUSION: This study identified the first evidence of an association of a miRNA-related variant with risk of ESCC in an Iranian cohort. PEX6 rs1129186 may not modulate the risk of ESCC in the cohort.
AIMS: Iran is located in the Asian esophageal cancer belt. It is a high-risk region for esophageal squamous cell carcinoma (ESCC). The extent to which genetic components, especially variants within miRNAs or their binding sites, contribute to risk of ESCC in the region is not yet fully understood. Herein, tests were done on an Iranian cohort to evaluate the association of miRNA-related polymorphisms in miR-423 (rs6505162) and peroxisomal biogenesis factor 6 (PEX6) (rs1129186 within a miR-149-5p-binding site) with the risk of ESCC risk. METHODS: This study recruited 200 ESCC patients and 300 healthy individuals. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism method. Target genes and biological processes that are regulated by miR-423 and may be affected by a change in miR-423 expression were identified by in silico analysis. RESULTS: Logistic regression analyses revealed an association between rs6505162 and ESCC, assuming codominant (AA vs. CC, odds ratios, OR [95% confidence interval, CI]: 0.32 [0.15-0.69], p-value: 0.0076), recessive (AA vs. CC+CA, OR [95% CI]: 0.35 [0.16-0.73], p-value: 0.0027), and log-additive models (OR [95% CI]: 0.69 [0.52-0.91], p-value: 0.0084). No significant association was observed for PEX6rs1129186. In silico analyses revealed several genes and biological processes that are regulated by miR-423 in ESCC. CONCLUSION: This study identified the first evidence of an association of a miRNA-related variant with risk of ESCC in an Iranian cohort. PEX6rs1129186 may not modulate the risk of ESCC in the cohort.
Authors: Muhammad Sohail Khan; Bashir Rahman; Taqweem Ul Haq; Fazal Jalil; Bilal Muhammad Khan; Saleh N Maodaa; Saleh A Al-Farraj; Hamed A El-Serehy; Aftab Ali Shah Journal: Genes (Basel) Date: 2021-04-28 Impact factor: 4.096
Authors: Imadeldin Elfaki; Rashid Mir; Mohammad Muzaffar Mir; Faisel M AbuDuhier; Abdullatif Taha Babakr; Jameel Barnawi Journal: J Pers Med Date: 2019-11-25