Sulaiman R Hamarneh1, Byeong-Moo Kim2, Kanakaraju Kaliannan1, Sara A Morrison1, Tyler J Tantillo1, Qingsong Tao1, Mussa M Rafat Mohamed1, Juan M Ramirez1, Aaron Karas1, Wei Liu1, Dong Hu1, Abeba Teshager1, Sarah Shireen Gul1, Konstantinos P Economopoulos1, Atul K Bhan3, Madhu S Malo1, Michael Y Choi4,5, Richard A Hodin6. 1. Department of Surgery, Harvard Medical School, Massachusetts General Hospital, 15 Parkman Street, Boston, MA, 02114, USA. 2. Gastrointestinal Unit, Department of Medicine, Harvard Medical School, Massachusetts General Hospital, 55 Fruit Street, Boston, MA, 02114, USA. 3. Department of Pathology, Harvard Medical School, Massachusetts General Hospital, Boston, MA, 02114, USA. 4. Gastrointestinal Unit, Department of Medicine, Harvard Medical School, Massachusetts General Hospital, 55 Fruit Street, Boston, MA, 02114, USA. mchoi@mgh.harvard.edu. 5. Harvard Stem Cell Institute, Cambridge, MA, 02138, USA. mchoi@mgh.harvard.edu. 6. Department of Surgery, Harvard Medical School, Massachusetts General Hospital, 15 Parkman Street, Boston, MA, 02114, USA. rhodin@mgh.harvard.edu.
Abstract
BACKGROUND AND AIMS: Bacterially derived factors from the gut play a major role in the activation of inflammatory pathways in the liver and in the pathogenesis of alcoholic liver disease. The intestinal brush-border enzyme intestinal alkaline phosphatase (IAP) detoxifies a variety of bacterial pro-inflammatory factors and also functions to preserve gut barrier function. The aim of this study was to investigate whether oral IAP supplementation could protect against alcohol-induced liver disease. METHODS: Mice underwent acute binge or chronic ethanol exposure to induce alcoholic liver injury and steatosis ± IAP supplementation. Liver tissue was assessed for biochemical, inflammatory, and histopathological changes. An ex vivo co-culture system was used to examine the effects of alcohol and IAP treatment in regard to the activation of hepatic stellate cells and their role in the development of alcoholic liver disease. RESULTS: Pretreatment with IAP resulted in significantly lower serum alanine aminotransferase compared to the ethanol alone group in the acute binge model. IAP treatment attenuated the development of alcohol-induced fatty liver, lowered hepatic pro-inflammatory cytokine and serum LPS levels, and prevented alcohol-induced gut barrier dysfunction. Finally, IAP ameliorated the activation of hepatic stellate cells and prevented their lipogenic effect on hepatocytes. CONCLUSIONS: IAP treatment protected mice from alcohol-induced hepatotoxicity and steatosis. Oral IAP supplementation could represent a novel therapy to prevent alcoholic-related liver disease in humans.
BACKGROUND AND AIMS: Bacterially derived factors from the gut play a major role in the activation of inflammatory pathways in the liver and in the pathogenesis of alcoholic liver disease. The intestinal brush-border enzyme intestinal alkaline phosphatase (IAP) detoxifies a variety of bacterial pro-inflammatory factors and also functions to preserve gut barrier function. The aim of this study was to investigate whether oral IAP supplementation could protect against alcohol-induced liver disease. METHODS:Mice underwent acute binge or chronic ethanol exposure to induce alcoholic liver injury and steatosis ± IAP supplementation. Liver tissue was assessed for biochemical, inflammatory, and histopathological changes. An ex vivo co-culture system was used to examine the effects of alcohol and IAP treatment in regard to the activation of hepatic stellate cells and their role in the development of alcoholic liver disease. RESULTS: Pretreatment with IAP resulted in significantly lower serum alanine aminotransferase compared to the ethanol alone group in the acute binge model. IAP treatment attenuated the development of alcohol-induced fatty liver, lowered hepatic pro-inflammatory cytokine and serum LPS levels, and prevented alcohol-induced gut barrier dysfunction. Finally, IAP ameliorated the activation of hepatic stellate cells and prevented their lipogenic effect on hepatocytes. CONCLUSIONS:IAP treatment protected mice from alcohol-induced hepatotoxicity and steatosis. Oral IAP supplementation could represent a novel therapy to prevent alcoholic-related liver disease in humans.
Authors: M S Malo; S Nasrin Alam; G Mostafa; S J Zeller; P V Johnson; N Mohammad; K T Chen; A K Moss; S Ramasamy; A Faruqui; S Hodin; P S Malo; F Ebrahimi; B Biswas; S Narisawa; J L Millán; H S Warren; J B Kaplan; C L Kitts; E L Hohmann; R A Hodin Journal: Gut Date: 2010-11 Impact factor: 23.059
Authors: Sulaiman R Hamarneh; Mussa M Rafat Mohamed; Konstantinos P Economopoulos; Sara A Morrison; Tanit Phupitakphol; Tyler J Tantillo; Sarah S Gul; Mohammad Hadi Gharedaghi; Qingsong Tao; Kanakaraju Kaliannan; Sonoko Narisawa; José L Millán; Gwendolyn M van der Wilden; Peter J Fagenholz; Madhu S Malo; Richard A Hodin Journal: Ann Surg Date: 2014-10 Impact factor: 12.969
Authors: Florian Kühn; Fatemeh Adiliaghdam; Paul M Cavallaro; Sulaiman R Hamarneh; Amy Tsurumi; Raza S Hoda; Alexander R Munoz; Yashoda Dhole; Juan M Ramirez; Enyu Liu; Robin Vasan; Yang Liu; Ehsan Samarbafzadeh; Rocio A Nunez; Matthew Z Farber; Vanita Chopra; Madhu S Malo; Laurence G Rahme; Richard A Hodin Journal: JCI Insight Date: 2020-03-26
Authors: Piero Portincasa; Leonilde Bonfrate; Mohamad Khalil; Maria De Angelis; Francesco Maria Calabrese; Mauro D'Amato; David Q-H Wang; Agostino Di Ciaula Journal: Biomedicines Date: 2021-12-31