Literature DB >> 2842234

Plasmid vectors with multiple cloning sites and cat-reporter gene for promoter cloning and analysis in animal cells.

T Frebourg1, O Brison.   

Abstract

We have constructed two vectors, pGCAT-A and pGCAT-C, designed to facilitate the construction of recombinant plasmids containing the bacterial gene (cat) coding for chloramphenicol acetyltransferase (CAT) under the control of eukaryotic promoter and/or enhancer elements. The cat gene was inserted downstream from a multiple cloning site (MCS) region with eleven unique restriction sites. The MCS region is in opposite orientation in the two vectors. The CAT activity of control extracts from cells transfected with pGCAT-A or pGCAT-C is very low. Insertion of the viral SV40 early promoter into one of the sites of the MCS region of pGCAT-A or pGCAT-C results in a 30- to 400-fold stimulation of the CAT activity.

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Year:  1988        PMID： 2842234     DOI： 10.1016/0378-1119(88)90468-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  15 in total

1.  Identification of a cis acting element responsible for muscle specific expression of the c-mos protooncogene.

Authors:  J L Lenormand; M Guillier; S A Leibovitch
Journal:  Nucleic Acids Res       Date:  1993-02-11       Impact factor: 16.971

2.  DNA recognition by splicing variants of the Wilms' tumor suppressor, WT1.

Authors:  I A Drummond; H D Rupprecht; P Rohwer-Nutter; J M Lopez-Guisa; S L Madden; F J Rauscher; V P Sukhatme
Journal:  Mol Cell Biol       Date:  1994-06       Impact factor: 4.272

3.  Overexpression of ERBB2 in human mammary epithelial cells signals inhibition of transcription of the E-cadherin gene.

Authors:  B D'souza; J Taylor-Papadimitriou
Journal:  Proc Natl Acad Sci U S A       Date:  1994-07-19       Impact factor: 11.205

4.  Specific binding of high-mobility-group I (HMGI) protein and histone H1 to the upstream AT-rich region of the murine beta interferon promoter: HMGI protein acts as a potential antirepressor of the promoter.

Authors:  E Bonnefoy; M T Bandu; J Doly
Journal:  Mol Cell Biol       Date:  1999-04       Impact factor: 4.272

5.  Endothelial cell-specific transcriptional targeting from a hybrid long terminal repeat retrovirus vector containing human prepro-endothelin-1 promoter sequences.

Authors:  U Jäger; Y Zhao; C D Porter
Journal:  J Virol       Date:  1999-12       Impact factor: 5.103

6.  Regulatory sequences of duck hepatitis B virus C gene transcription.

Authors:  R Schneider; H Will
Journal:  J Virol       Date:  1991-11       Impact factor: 5.103

7.  Cloning of a functional Burkitt's lymphoma polypeptide-binding protein/78 kDa glucose-regulated protein (BiP/GRP78) gene promoter by the polymerase chain reaction, and its interaction with inducible cellular factors.

Authors:  C C Chao; W C Yam; L K Chen; S Lin-Chao
Journal:  Biochem J       Date:  1992-09-01       Impact factor: 3.857

8.  The role of the 5'-flanking region in the cell-specific transcription of the human von Willebrand factor gene.

Authors:  V Ferreira; Z Assouline; J L Schwachtgen; B R Bahnak; D Meyer; D Kerbiriou-Nabias
Journal:  Biochem J       Date:  1993-08-01       Impact factor: 3.857

9.  Inhibition of gene expression by triple helix-directed DNA cross-linking at specific sites.

Authors:  M Grigoriev; D Praseuth; A L Guieysse; P Robin; N T Thuong; C Hélène; A Harel-Bellan
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-15       Impact factor: 11.205

10.  Transient activity assays of the Trypanosoma brucei variant surface glycoprotein gene promoter: control of gene expression at the posttranscriptional level.

Authors:  D Jefferies; P Tebabi; E Pays
Journal:  Mol Cell Biol       Date:  1991-01       Impact factor: 4.272
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