Chongqi Sun1, Pei Ma1, Yanfen Wang2, Weitao Liu3, Qinnan Chen4, Yutian Pan1, Chenhui Zhao1, Yingchen Qian3, Jie Liu1, Wei Li5,6, Yongqian Shu7,8,9. 1. Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, No.300 Guangzhou Road, Nanjing, People's Republic of China. 2. Department of Pathology, Yangzhou No.1 People's Hospital, Yangzhou, People's Republic of China. 3. Department of Pathology, Nanjing Medical University, Nanjing, People's Republic of China. 4. Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China. 5. Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, No.300 Guangzhou Road, Nanjing, People's Republic of China. real.lw@163.com. 6. Department of Medical Oncology, Affiliated Sir Run Run Hospital of Nanjing Medical University, Nanjing, People's Republic of China. real.lw@163.com. 7. Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, No.300 Guangzhou Road, Nanjing, People's Republic of China. yongqianshu@hotmail.com. 8. Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, Nanjing, People's Republic of China. yongqianshu@hotmail.com. 9. Department of Medical Oncology, Affiliated Sir Run Run Hospital of Nanjing Medical University, Nanjing, People's Republic of China. yongqianshu@hotmail.com.
Abstract
BACKGROUND: Krüppel-like factors (KLFs) have been identified in multi-cancers and act as oncogenes or tumor suppressors. The function of KLF15, one member of KLFs, has not been well elucidated, especially in gastric cancer (GC). AIMS: This study was designed to investigate the prognostic value and biological functions of KLF15 in GC. METHODS: KLF15 protein expression in GC patients was evaluated by immunohistochemistry assays in 50 paired GC tissues and adjacent normal tissues, and correlations between KLF15 expression and clinicopathological characteristics and prognosis were analyzed. Then, we investigated the over-expression of KLF15 on cell proliferation and its mechanism in GC cells. RESULTS: KLF15 expression levels were significantly down-regulated in GC tissues compared to adjacent normal tissues. And KLF15 expression was negatively correlated with clinical stage, lymphatic metastasis, and distant metastasis. Furthermore, KLF15 expression could predict prognosis in patients with GC. Moreover, over-expression of KLF15 could inhibit cell proliferation partly via regulating CDKN1A/p21 and CDKN1C/p57. CONCLUSION: These findings demonstrate that KLF15 plays a significant role in GC progression and could be a therapeutic target for GC.
BACKGROUND: Krüppel-like factors (KLFs) have been identified in multi-cancers and act as oncogenes or tumor suppressors. The function of KLF15, one member of KLFs, has not been well elucidated, especially in gastric cancer (GC). AIMS: This study was designed to investigate the prognostic value and biological functions of KLF15 in GC. METHODS:KLF15 protein expression in GC patients was evaluated by immunohistochemistry assays in 50 paired GC tissues and adjacent normal tissues, and correlations between KLF15 expression and clinicopathological characteristics and prognosis were analyzed. Then, we investigated the over-expression of KLF15 on cell proliferation and its mechanism in GC cells. RESULTS:KLF15 expression levels were significantly down-regulated in GC tissues compared to adjacent normal tissues. And KLF15 expression was negatively correlated with clinical stage, lymphatic metastasis, and distant metastasis. Furthermore, KLF15 expression could predict prognosis in patients with GC. Moreover, over-expression of KLF15 could inhibit cell proliferation partly via regulating CDKN1A/p21 and CDKN1C/p57. CONCLUSION: These findings demonstrate that KLF15 plays a significant role in GC progression and could be a therapeutic target for GC.
Authors: Sara E Meyer; Jason R Hasenstein; Avinash Baktula; Chinavenmani S Velu; Yan Xu; Huajing Wan; Jeffrey A Whitsett; C Blake Gilks; H Leighton Grimes Journal: Am J Pathol Date: 2010-07-16 Impact factor: 4.307
Authors: Sharon Benzeno; Goutham Narla; Jorge Allina; George Z Cheng; Helen L Reeves; Michaela S Banck; Joseph A Odin; J Alan Diehl; Doris Germain; Scott L Friedman Journal: Cancer Res Date: 2004-06-01 Impact factor: 12.701