| Literature DB >> 28417367 |
Daniel J Cosgrove1, Nathan K Hepler2, Edward R Wagner2, Daniel M Durachko2.
Abstract
Expansins are proteins that loosen plant cell walls but lack enzymatic activity. Here, we describe two protocols tailored to measure the biomechanical activity of bacterial expansin. The first assay relies on weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.Keywords: Cellulose; Creep; Expansin; Filter paper; Plant cell wall; Wall loosening proteins
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Year: 2017 PMID： 28417367 DOI： 10.1007/978-1-4939-6899-2_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745