Marcos Mônico-Neto1,2, Murilo Dáttilo1,2, Daniel Araki Ribeiro3, Kil Sun Lee4, Marco Túlio de Mello1,5, Sergio Tufik1, Hanna Karen Moreira Antunes1,3,2. 1. a Department of Psychobiology. 2. d Laboratório Interdisciplinar em Fisiologia e Exercício - LAIFE , São Paulo , Brazil , and. 3. b Department of Biosciences. 4. c Department of Biochemistry , Universidade Federal de São Paulo , São Paulo , Brazil. 5. e School of Physical Education, Physiotherapy and Occupational Therapy, Universidade Federal de Minas Gerais , Belo Horizonte , Brazil.
Abstract
INTRODUCTION: The aim was observe the influence of sleep deprivation (SD) and sleep recovery on muscle regeneration process in rats submitted to cryolesion. METHODS: Thirty-two Wistar rats were randomly allocated in four groups: control (CTL), SD for 96 h (SD96), control plus sleep recovery period (CTL + R) and SD96h plus 96 h of sleep recovery (SD96 + R). The animals were submitted to muscle injury by cryolesioning, after to SD and sleep recovery. RESULTS: The major outcomes of this study were the reduction of muscular IGF-1 in both legs (injured and uninjured) and a delay in muscle regeneration process of animals submitted to SD compared to animals that slept, with increase connective tissue, inflammatory infiltrate and minor muscle fibers. CONCLUSIONS: SD impairs muscle regeneration in rats, moreover reduces muscular IGF-1 and sleep recovery was able to restore it to basal levels, but it was not enough to normalize the muscle regeneration.
INTRODUCTION: The aim was observe the influence of sleep deprivation (SD) and sleep recovery on muscle regeneration process in rats submitted to cryolesion. METHODS: Thirty-two Wistar rats were randomly allocated in four groups: control (CTL), SD for 96 h (SD96), control plus sleep recovery period (CTL + R) and SD96h plus 96 h of sleep recovery (SD96 + R). The animals were submitted to muscle injury by cryolesioning, after to SD and sleep recovery. RESULTS: The major outcomes of this study were the reduction of muscular IGF-1 in both legs (injured and uninjured) and a delay in muscle regeneration process of animals submitted to SD compared to animals that slept, with increase connective tissue, inflammatory infiltrate and minor muscle fibers. CONCLUSIONS:SD impairs muscle regeneration in rats, moreover reduces muscular IGF-1 and sleep recovery was able to restore it to basal levels, but it was not enough to normalize the muscle regeneration.
Entities:
Keywords:
IGF-1; muscle recovery; skeletal muscle; sleep loss
Authors: Diego E Vázquez; M Sol Balbuena; Fidel Chaves; Jacob Gora; Randolf Menzel; Walter M Farina Journal: Sci Rep Date: 2020-06-29 Impact factor: 4.379
Authors: Andressa Silva; Fernanda Veruska Narciso; João Paulo Rosa; Dayane Ferreira Rodrigues; Aline Ângela da Silva Cruz; Sérgio Tufik; Fernanda Viana; Jorge José Bichara; Sebastian Rafael Dias Pereira; Sidney Cavalcante da Silva; Marco Túlio De Mello Journal: Sleep Sci Date: 2019 Oct-Dec