Nasimudeen R Jabir1, Chelapram K Firoz1, Farid Ahmed2, Mohammad A Kamal1, Salwa Hindawi3, Ghazi A Damanhouri1, Hussein A Almehdar4, Shams Tabrez1. 1. a King Fahd Medical Research Center , King Abdulaziz University , Jeddah , Saudi Arabia. 2. b Center of Excellence in Genomic Medicine Research (CEGMR) , King Abdulaziz University , Jeddah , Saudi Arabia. 3. c Department of Haematology , King Abdulaziz University Hospital , Jeddah , Saudi Arabia. 4. d Department of Biology, Faculty of Science , King Abdulaziz University , Jeddah , Saudi Arabia.
Abstract
BACKGROUND: Natural killer (NK) cells are the potential modulators of inflammatory reactions that exert several unique biological effects and could lead to future adverse events of coronary artery disease (CAD). HYPOTHESIS: The purpose of this study was to find out the possible association of modulation in NK cell, TNK cells, T cells, B cells, and tumor necrosis factor alpha (TNF-α) in CAD patients and various forms of myocardial infarction. METHODS: The present study included total 190 subjects (98 confirmed CAD patients both men and women and 92 healthy control individuals). Serum concentration of TNF-α was measured by ELISA method. For the measurement of various immune cells, viz., NK cell, TNK cells, T cells, and B cells, flow-cytometric analysis was performed. RESULTS: A significant reduction by 15% (P < 0.001) in CD16/CD56 NK cells was observed in CAD patients. Moreover, non-ST segment elevation myocardial infarction (NSTEMI), ST segment elevation myocardial infarction (STEMI), unstable angina (UA), and combined UA + NSTEMI group also showed a significant decline in NK cells compared with control individuals. CD16/CD56/CD3 TNK cells showed a significant reduction in CAD, NSTEMI, STEMI, and UA categories. However, UA + NSTEMI group did not show any significant change in TNK cells. On the other hand, the level of TNF-α was found to be significantly elevated in CAD, STEMI, and UA groups. NSTEMI and combined UA + NSTEMI group did not show any significant change in TNF-α level. CONCLUSION: Current study provides an insight toward the association of immune cells and inflammation with CAD.
BACKGROUND: Natural killer (NK) cells are the potential modulators of inflammatory reactions that exert several unique biological effects and could lead to future adverse events of coronary artery disease (CAD). HYPOTHESIS: The purpose of this study was to find out the possible association of modulation in NK cell, TNK cells, T cells, B cells, and tumor necrosis factor alpha (TNF-α) in CAD patients and various forms of myocardial infarction. METHODS: The present study included total 190 subjects (98 confirmed CAD patients both men and women and 92 healthy control individuals). Serum concentration of TNF-α was measured by ELISA method. For the measurement of various immune cells, viz., NK cell, TNK cells, T cells, and B cells, flow-cytometric analysis was performed. RESULTS: A significant reduction by 15% (P < 0.001) in CD16/CD56 NK cells was observed in CAD patients. Moreover, non-ST segment elevation myocardial infarction (NSTEMI), ST segment elevation myocardial infarction (STEMI), unstable angina (UA), and combined UA + NSTEMI group also showed a significant decline in NK cells compared with control individuals. CD16/CD56/CD3 TNK cells showed a significant reduction in CAD, NSTEMI, STEMI, and UA categories. However, UA + NSTEMI group did not show any significant change in TNK cells. On the other hand, the level of TNF-α was found to be significantly elevated in CAD, STEMI, and UA groups. NSTEMI and combined UA + NSTEMI group did not show any significant change in TNF-α level. CONCLUSION: Current study provides an insight toward the association of immune cells and inflammation with CAD.
Entities:
Keywords:
CAD; NK cells; immune cells; inflammation; interleukins
Authors: Jordan Stillman; Alicia Martin; Maria-Jose Miguez; H Reginald McDaniel; Janet Konefal; Judi M Woolger; John E Lewis Journal: J Clin Transl Res Date: 2020-01-29
Authors: Yvonne Baumer; Cristhian A Gutierrez-Huerta; Ankit Saxena; Pradeep K Dagur; Steven D Langerman; Kosuke Tamura; Joniqua N Ceasar; Marcus R Andrews; Valerie Mitchell; Billy S Collins; Quan Yu; Heather L Teague; Martin P Playford; Christopher K E Bleck; Nehal N Mehta; J Philip McCoy; Tiffany M Powell-Wiley Journal: J Transl Med Date: 2020-01-17 Impact factor: 5.531