Literature DB >> 2841292

A Bacillus thuringiensis subsp. israelensis gene encoding a 125-kilodalton larvicidal polypeptide is associated with inverted repeat sequences.

C Bourgouin1, A Delécluse, J Ribier, A Klier, G Rapoport.   

Abstract

A gene encoding a 125-kilodalton (kDa) mosquitocidal delta-endotoxin was cloned from the 72-MDa resident plasmid of Bacillus thuringiensis subsp. israelensis. This gene is similar in its 3' region to the gene encoding the 135-kDa protein previously cloned (C. Bourgouin, A. Klier, and G. Rapoport, Mol. Gen. Genet. 205:390-397, 1986). Escherichia coli recombinant clones harboring the 125-kDa gene were toxic to larvae of the three mosquito species Aedes aegypti, Anopheles stephensi, and Culex pipiens. In addition, the B. thuringiensis subsp. israelensis DNA fragment carrying the 125-kDa protein gene contains two sets of inverted repeat sequences, identified either by the S1 nuclease method or by electron microscopic observation. The structural organization of inverted repeat sequences and of the 125-kDa gene was analyzed and suggests that this B. thuringiensis subsp. israelensis delta-endotoxin gene is located within a transposable element.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 2841292      PMCID： PMC211331          DOI： 10.1128/jb.170.8.3575-3583.1988

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  38 in total

1.  Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.

Authors:  J C Alwine; D J Kemp; B A Parker; J Reiser; J Renart; G R Stark; G M Wahl
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors:  G M Wahl; M Stern; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

4.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 6.  Transposable elements in prokaryotes.

Authors:  N Kleckner
Journal:  Annu Rev Genet       Date:  1981       Impact factor: 16.830

7.  A rapid boiling method for the preparation of bacterial plasmids.

Authors:  D S Holmes; M Quigley
Journal:  Anal Biochem       Date:  1981-06       Impact factor: 3.365

8.  A comparison of protein crystal subunit sizes in Bacillus thuringiensis.

Authors:  D M Calabrese; K W Nickerson; L C Lane
Journal:  Can J Microbiol       Date:  1980-08       Impact factor: 2.419

9.  Multiplicity of delta-endotoxin genes with different insecticidal specificities in Bacillus thuringiensis aizawai 7.29.

Authors:  V Sanchis; D Lereclus; G Menou; J Chaufaux; M M Lecadet
Journal:  Mol Microbiol       Date:  1988-05       Impact factor: 3.501

10.  Isolation of inverted repeat sequences, including IS1, IS2, and IS3, in Escherichia coli plasmids.

Authors:  H Ohtsubo; E Ohtsubo
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205
View more
  19 in total

1.  A new insertion variant, IS231I, isolated from a mosquito-specific strain of Bacillus thuringiensis.

Authors:  Akira Ohgushi; Hiroyuki Saitoh; Naoya Wasano; Michio Ohba
Journal:  Curr Microbiol       Date:  2005-06-27       Impact factor: 2.188

2.  Expression of cryIVA and cryIVB Genes, Independently or in Combination, in a Crystal-Negative Strain of Bacillus thuringiensis subsp. israelensis.

Authors:  A Delécluse; S Poncet; A Klier; G Rapoport
Journal:  Appl Environ Microbiol       Date:  1993-11       Impact factor: 4.792

3.  Restriction map of the 125-kilobase plasmid of Bacillus thuringiensis subsp. israelensis carrying the genes that encode delta-endotoxins active against mosquito larvae.

Authors:  E Ben-Dov; M Einav; N Peleg; S Boussiba; A Zaritsky
Journal:  Appl Environ Microbiol       Date:  1996-09       Impact factor: 4.792

4.  Structural and genetic organization of IS232, a new insertion sequence of Bacillus thuringiensis.

Authors:  G Menou; J Mahillon; M M Lecadet; D Lereclus
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

5.  Role of cyclic-AMP-dependent protein kinase in catabolite inactivation of the glucose and galactose transporters in Saccharomyces cerevisiae.

Authors:  J Ramos; V P Cirillo
Journal:  J Bacteriol       Date:  1989-06       Impact factor: 3.490

6.  Deletion by in vivo recombination shows that the 28-kilodalton cytolytic polypeptide from Bacillus thuringiensis subsp. israelensis is not essential for mosquitocidal activity.

Authors:  A Delécluse; J F Charles; A Klier; G Rapoport
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

Review 7.  Bacillus thuringiensis and its pesticidal crystal proteins.

Authors:  E Schnepf; N Crickmore; J Van Rie; D Lereclus; J Baum; J Feitelson; D R Zeigler; D H Dean
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

8.  Mosquito larvicidal activity of Escherichia coli with combinations of genes from Bacillus thuringiensis subsp. israelensis.

Authors:  E Ben-Dov; S Boussiba; A Zaritsky
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

Review 9.  Insecticidal crystal proteins of Bacillus thuringiensis.

Authors:  H Höfte; H R Whiteley
Journal:  Microbiol Rev       Date:  1989-06

10.  Contribution of the 65-kilodalton protein encoded by the cloned gene cry19A to the mosquitocidal activity of Bacillus thuringiensis subsp. jegathesan.

Authors:  M L Rosso; A Delécluse
Journal:  Appl Environ Microbiol       Date:  1997-11       Impact factor: 4.792
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.