Ola Galal El-Farghali1, Mohamed Sami El-Chimi1, Heba Salah El-Abd2, Eman El-Desouky3. 1. a Neonatology Division, Department of Pediatrics, Faculty of Medicine , Ain Shams University , Cairo , Egypt. 2. b Genetics Division, Department of Pediatrics, Faculty of Medicine , Ain Shams University , Cairo , Egypt. 3. c Department of Epidemiology and Biostatistics , National Cancer Institute, Cairo University , Cairo , Egypt.
Abstract
OBJECTIVE: To estimate cord blood amino acid and acylcarnitine levels in term newborns exposed to perinatal asphyxia. MATERIALS AND METHODS: We studied 45 asphyxiated term newborns (cases) and 20 gestational age-matched healthy newborns (control). 16 cases developed HIE according to clinical scoring and amplitude-integrated electroencephalography. Asphyxiated cases were accordingly subdivided into: HIE group (n = 16) and Asphyxia group (n = 29). Amino acid and acylcarnitine levels were measured in cord blood dried spot samples from all newborns using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Data were analyzed using one-way ANOVA with post hoc test and MetaboAnalyst-2. RESULTS: Distinct metabolite alterations were detected in cases versus control, in HIE versus Asphyxia, and in Survivors within HIE group (n = 6) versus nonsurvivors (n = 10). Principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA) showed increased levels of methionine and certain acylcarnitines, but reduced levels of ornithine, histidine, and arginine. Metabolite set enrichment analysis (MSEA); compared to KEGG library metabolite sets, identified some disorders with similar metabolomic derangements. CONCLUSIONS: We report UPLC-MS detectable alterations of amino acids and acylcarnitines in asphyxiated newborns at birth, that can serve as early diagnostic bedside biomarkers for HIE and predictors for its short-term outcome, and in the near future, as therapeutic targets.
OBJECTIVE: To estimate cord blood amino acid and acylcarnitine levels in term newborns exposed to perinatal asphyxia. MATERIALS AND METHODS: We studied 45 asphyxiated term newborns (cases) and 20 gestational age-matched healthy newborns (control). 16 cases developed HIE according to clinical scoring and amplitude-integrated electroencephalography. Asphyxiated cases were accordingly subdivided into: HIE group (n = 16) and Asphyxia group (n = 29). Amino acid and acylcarnitine levels were measured in cord blood dried spot samples from all newborns using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Data were analyzed using one-way ANOVA with post hoc test and MetaboAnalyst-2. RESULTS: Distinct metabolite alterations were detected in cases versus control, in HIE versus Asphyxia, and in Survivors within HIE group (n = 6) versus nonsurvivors (n = 10). Principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA) showed increased levels of methionine and certain acylcarnitines, but reduced levels of ornithine, histidine, and arginine. Metabolite set enrichment analysis (MSEA); compared to KEGG library metabolite sets, identified some disorders with similar metabolomic derangements. CONCLUSIONS: We report UPLC-MS detectable alterations of amino acids and acylcarnitines in asphyxiated newborns at birth, that can serve as early diagnostic bedside biomarkers for HIE and predictors for its short-term outcome, and in the near future, as therapeutic targets.
Authors: K Sarafidis; N Efstathiou; O Begou; V Soubasi; E Agakidou; E Gika; G Theodoridis; V Drossou Journal: Hippokratia Date: 2017 Apr-Jun Impact factor: 0.471