| Literature DB >> 28409452 |
Janet Koster1, Hans R Waterham2.
Abstract
Functional studies with primary human skin fibroblasts from patients with a peroxisomal disorder often require efficient transfection with plasmids to correct the genetic defect or to express heterologous reporter proteins. Here, we describe a protocol we commonly use for efficient nonviral transfection of primary human skin fibroblasts.Entities:
Keywords: Functional complementation; Nucleofection; Primary cells; Transfection
Mesh：
Year: 2017 PMID： 28409452 DOI： 10.1007/978-1-4939-6937-1_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745