Literature DB >> 28409450

Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and De Novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labeled Docosanoic Acid.

Malu-Clair van de Beek1, Inge M E Dijkstra1, Stephan Kemp2.   

Abstract

Peroxisomes are present in virtually every eukaryotic cell type with the exception of the mature erythrocyte. In higher eukaryotes, one of the main functions of peroxisomes is lipid metabolism by means of beta-oxidation of very long-chain fatty acids (VLCFA; ≥22 carbon atoms). A dysfunction in peroxisomal VLCFA beta-oxidation results in elevated VLCFA levels in cells, tissue, and plasma. Here, we describe a straightforward and sensitive method to measure peroxisomal beta-oxidation capacity in living cells using stable-isotope labeled docosanoic acid (D3-C22:0).

Entities:  

Keywords:  Beta-oxidation; Elongation; Fatty acids; Peroxisome; Stable-isotope; Very-long-chain fatty acids

Mesh：

Substances：

Year:  2017        PMID： 28409450     DOI： 10.1007/978-1-4939-6937-1_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.

Authors:  Hongsuk Park; Anyuan He; Min Tan; Jordan M Johnson; John M Dean; Terri A Pietka; Yali Chen; Xiangyu Zhang; Fong-Fu Hsu; Babak Razani; Katsuhiko Funai; Irfan J Lodhi
Journal:  J Clin Invest       Date:  2019-01-14       Impact factor: 14.808

2.  Pkd1 Mutation Has No Apparent Effects on Peroxisome Structure or Lipid Metabolism.

Authors:  Takeshi Terabayashi; Luis F Menezes; Fang Zhou; Hongyi Cai; Peter J Walter; Hugo M Garraffo; Gregory G Germino
Journal:  Kidney360       Date:  2021-07-16

3.  Metabolic rerouting via SCD1 induction impacts X-linked adrenoleukodystrophy.

Authors:  Quentin Raas; Malu-Clair van de Beek; Sonja Forss-Petter; Inge Me Dijkstra; Abigail Deschiffart; Briana C Freshner; Tamara J Stevenson; Yorrick Rj Jaspers; Liselotte Nagtzaam; Ronald Ja Wanders; Michel van Weeghel; Joo-Yeon Engelen-Lee; Marc Engelen; Florian Eichler; Johannes Berger; Joshua L Bonkowsky; Stephan Kemp
Journal:  J Clin Invest       Date:  2021-04-15       Impact factor: 14.808

4.  Adrenoleukodystrophy Newborn Screening in the Netherlands (SCAN Study): The X-Factor.

Authors:  Rinse W Barendsen; Inge M E Dijkstra; Wouter F Visser; Mariëlle Alders; Jet Bliek; Anita Boelen; Marelle J Bouva; Saskia N van der Crabben; Ellen Elsinghorst; Ankie G M van Gorp; Annemieke C Heijboer; Mandy Jansen; Yorrick R J Jaspers; Henk van Lenthe; Ingrid Metgod; Christiaan F Mooij; Elise H C van der Sluijs; A S Paul van Trotsenburg; Rendelien K Verschoof-Puite; Frédéric M Vaz; Hans R Waterham; Frits A Wijburg; Marc Engelen; Eugènie Dekkers; Stephan Kemp
Journal:  Front Cell Dev Biol       Date:  2020-06-17

5.  Biochemical Studies in Fibroblasts to Interpret Variants of Unknown Significance in the ABCD1 Gene.

Authors:  Stephanie I W van de Stadt; Petra A W Mooyer; Inge M E Dijkstra; Conny J M Dekker; Divya Vats; Moin Vera; Maura R Z Ruzhnikov; Keith van Haren; Nelson Tang; Klaas Koop; Michel A Willemsen; Joannie Hui; Frédéric M Vaz; Merel S Ebberink; Marc Engelen; Stephan Kemp; Sacha Ferdinandusse
Journal:  Genes (Basel)       Date:  2021-11-30       Impact factor: 4.096
  5 in total

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