| Literature DB >> 28405840 |
Xiao Liang1,2,3,4, Qing Chen5,6,7,8, Hui Lu1,2,3,4, Chunling Wu1,2,3,4, Fuping Lu1,2,3,4, Jihong Tang1,2,3,4.
Abstract
In order to study the function of peroxidase (POD) and polyphenol oxidase (PPO) in cassava resistance to spider mites, we tested the changes of transcription levels and activities of these two protective enzymes in both cassava and Tetranychus urticae (=T. cinnabarinus) during the interaction. The results showed that after damage of the mite-susceptible cassava cultivar BRA900 by T. urticae for 1 and 8 days, the transcription levels of MePOD and MePPO and the activities of POD and PPO showed no significant difference compared with those in undamaged leaves. However, the corresponding transcription levels and activities in 1- and 8-day-damaged leaves of mite-resistant cassava cultivar C1115 increased to a significant level of approximately twofold. When T. urticae fed on BRA900 for 1 and 8 days, the transcription levels of TcPPO and TcPOD and the activities of PPO and POD showed no significant difference compared with those before feeding. However, the corresponding transcription levels and activities of these two protective enzymes in T. urticae feeding on C1115 significantly decreased by about half. This study preliminarily validates the function of POD and PPO in cassava resistance to T. urticae, and provides candidate gene resource for molecular breeding of spider mite-resistant cassava.Entities:
Keywords: Cassava resistance; Peroxidase; Polyphenol oxidase; Tetranychus urticae
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Year: 2017 PMID： 28405840 DOI： 10.1007/s10493-017-0125-y
Source DB: PubMed Journal: Exp Appl Acarol ISSN： 0168-8162 Impact factor: 2.132