Literature DB >> 2840356

Cloning and expression of two cellulase genes of Clostridium cellulolyticum in Escherichia coli.

E Faure1, C Bagnara, A Belaich, J P Belaich.   

Abstract

Two cellulase genes isolated from Clostridium cellulolyticum strain ATCC3519 were cloned in Escherichia coli using plasmid pACYC184. Plasmids pB52 and pB43 were isolated from the transformants producing carboxymethylcellulase (CMCase) and the two cloned CMCase-coding genes were found to be included in two EcoRI fragments of 5.7 kb and 2.6 kb, respectively. These two genes showed no homology. The CMCase-coding genes were found to be contained in a 1.8-kb KpnI-HindIII fragment and a 2.05-kb HindIII-PvuII fragment of the DNA donor strain. Expression of these genes in E. coli was found not to depend on their orientation in the cloning vector. Hybridization experiments between these two fragments and Clostridium thermocellum NCIB10682 DNA fragments carrying genes celA, celB, celC and celD were carried out and some homologies were detected.

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Year:  1988        PMID： 2840356     DOI： 10.1016/0378-1119(88)90416-7

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  11 in total

Review 1.  Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.

Authors:  N R Gilkes; B Henrissat; D G Kilburn; R C Miller; R A Warren
Journal:  Microbiol Rev       Date:  1991-06

2.  Insertion of endocellulase catalytic domains into thermostable consensus ankyrin scaffolds: effects on stability and cellulolytic activity.

Authors:  Eva S Cunha; Christine L Hatem; Doug Barrick
Journal:  Appl Environ Microbiol       Date:  2013-08-23       Impact factor: 4.792

3.  Colonization of Crystalline Cellulose by Clostridium cellulolyticum ATCC 35319.

Authors:  E Gelhaye; A Gehin; H Petitdemange
Journal:  Appl Environ Microbiol       Date:  1993-09       Impact factor: 4.792

4.  Characterization of the cellulolytic complex (cellulosome) produced by Clostridium cellulolyticum.

Authors:  L Gal; S Pages; C Gaudin; A Belaich; C Reverbel-Leroy; C Tardif; J P Belaich
Journal:  Appl Environ Microbiol       Date:  1997-03       Impact factor: 4.792

5.  The catalytic domain of endoglucanase A from Clostridium cellulolyticum: effects of arginine 79 and histidine 122 mutations on catalysis.

Authors:  A Belaich; H P Fierobe; D Baty; B Busetta; C Bagnara-Tardif; C Gaudin; J P Belaich
Journal:  J Bacteriol       Date:  1992-07       Impact factor: 3.490

6.  Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase.

Authors:  L Zhou; G P Xue; C G Orpin; G W Black; H J Gilbert; G P Hazlewood
Journal:  Biochem J       Date:  1994-01-15       Impact factor: 3.857

7.  Clostridium cellulolyticum Viability and Sporulation under Cellobiose Starvation Conditions.

Authors:  A Gehin; E Gelhaye; G Raval; H Petitdemange
Journal:  Appl Environ Microbiol       Date:  1995-03       Impact factor: 4.792

8.  Biochemical properties of a beta-xylosidase from Clostridium cellulolyticum.

Authors:  S Saxena; H P Fierobe; C Gaudin; F Guerlesquin; J P Belaich
Journal:  Appl Environ Microbiol       Date:  1995-09       Impact factor: 4.792

9.  Purification and properties of two truncated endoglucanases produced in Escherichia coli harbouring Clostridium cellulolyticum endoglucanase gene celCCD.

Authors:  S Shima; Y Igarashi; T Kodama
Journal:  Appl Microbiol Biotechnol       Date:  1993-03       Impact factor: 4.813

10.  Molecular cloning, expression, and characterization of endo-beta-1,4-glucanase genes from Bacillus polymyxa and Bacillus circulans.

Authors:  S D Baird; D A Johnson; V L Seligy
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490
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