Thais Inácio de Carvalho1, Paulo Cezar Novais2, Fermino Sanches Lizarte3, Renata Danielle Sicchieri4, Marcella Suelma Torrecillas Rosa5, Camila Albuquerque Mello de Carvalho4, Daniela Pretti da Cunha Tirapelli6, Fernanda Maris Peria7, José Joaquim Ribeiro da Rocha8, Omar Féres9. 1. PhD, Postgraduate Program in Clinical Surgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, Universidade de São Paulo (USP), Brazil. Acquisition and interpretation of data, manuscript writing. 2. PhD, Department of Health Sciences, Universidade de Marília (UNIMAR), Pos-doctoral Fellow, Postgraduate Program in Clinical Surgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Technical procedures, manuscript writing. 3. Pos-doctoral Fellow, Postgraduate Program in Clinical Surgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Technical procedures, manuscript writing. 4. Fellow PhD degree, Postgraduate Program in Clinical Surgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Manuscript writing. 5. Fellow Master degree, Postgraduate Program in Clinical Surgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Manuscript writing. 6. PhD, Department of Surgery and Anatomy, Postgraduate Program in Clinical Surgery, School of Medicine of Ribeirao Preto, USP, Brazil. Scientific and intellectual content of the study. 7. PhD, Department of Medical Clinical, Oncology Program, School of Medicine of Ribeirão Preto, USP, Brazil. Scientific and intellectual content of the study. 8. Associate Professor, Coloproctology Division, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Scientific and intellectual content of the study. 9. Associate Professor, Coloproctology Division, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Concept, design, intellectual and scientific content of the study; supervision of all phases of the study, critical revision.
Abstract
PURPOSE: : To evaluate the expression of EGFR, KRAS genes, microRNAs-21 and 203 in colon and rectal cancer samples, correlated with their age at diagnosis, histological subtype, value of pretreatment CEA, TNM staging and clinical outcome. METHODS: : Expression of genes and microRNAs by real time PCR in tumor and non-tumor samples obtained from surgical treatment of 50 patients. RESULTS: : An increased expression of microRNAs-21 and 203 in tumor samples in relation to non-tumor samples was found. There was no statistically significant difference between the expression of these genes and microRNAs when compared to age at diagnosis and histological subtype. The EGFR gene showed higher expression in relation to the value of CEA diagnosis. The expression of microRNA-203 was progressively lower in relation to the TNM staging and was higher in the patient group in clinical remission. CONCLUSIONS: : The therapy of colon and rectum tumors based on microRNAs remains under investigation reserving huge potential for future applications and clinical interventions in conjunction with existing therapies. We expect, based on the exposed data, to stimulate the development of new therapeutic possibilities, making the treatment of these tumors more effective.
PURPOSE: : To evaluate the expression of EGFR, KRAS genes, microRNAs-21 and 203 in colon and rectal cancer samples, correlated with their age at diagnosis, histological subtype, value of pretreatment CEA, TNM staging and clinical outcome. METHODS: : Expression of genes and microRNAs by real time PCR in tumor and non-tumor samples obtained from surgical treatment of 50 patients. RESULTS: : An increased expression of microRNAs-21 and 203 in tumor samples in relation to non-tumor samples was found. There was no statistically significant difference between the expression of these genes and microRNAs when compared to age at diagnosis and histological subtype. The EGFR gene showed higher expression in relation to the value of CEA diagnosis. The expression of microRNA-203 was progressively lower in relation to the TNM staging and was higher in the patient group in clinical remission. CONCLUSIONS: : The therapy of colon and rectum tumors based on microRNAs remains under investigation reserving huge potential for future applications and clinical interventions in conjunction with existing therapies. We expect, based on the exposed data, to stimulate the development of new therapeutic possibilities, making the treatment of these tumors more effective.