Rita de Cássia Silva de Oliveira1, Marcus Vinicius Henriques Brito2, Rubens Fernando Gonçalves Ribeiro3, Leonam Oliver Durval Oliveira4, Andrew Moraes Monteiro4, Fernando Mateus Viegas Brandão4, Lainy Carollyne da Costa Cavalcante5, Eduardo Henrique Herbster Gouveia5, Higor Yuri Bezerra Henriques5. 1. PhD, Full Professor, Department of Pharmacology, Universidade Estadual do Pará (UEPA), Belem-PA, Brazil. Conception, design and scientific content of the study; critical revision. 2. PhD, Full Professor, Department of Experimental Surgery, UEPA, Belem-PA, Brazil. Conception, design and scientific content of the study; critical revision. 3. Master, Postgraduate Program in Surgery and Experimental Research, UEPA, Belem-PA, Brazil. Statistical analysis, manuscript preparation, English version. 4. Graduate student, School of Medicine, UEPA, Belem-PA, Brazil. Technical procedures, interpretation of data, manuscript preparation. 5. Graduate student, School of Medicine, Centro Universitário do Pará (CESUPA), Belem-PA, Brazil. Technical procedures, interpretation of data, manuscript preparation.
Abstract
PURPOSE: : To evaluate the effects of tramadol hydrochloride associated to remote ischemic perconditioning on oxidative stress. METHODS: : Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Tramadol group (T) treated with tramadol hydrochloride (40mg/kg); remote ischemic perconditioning + Tramadol group (Per+T) with both treatments. Oxidative stress was assessed after 24 hours of reperfusion. RESULTS: : Statistical differences were observed in MDA levels between I/R group with all groups (p<0.01), in addition there was difference between Tramadol with Sham, Per and Per+T groups (p<0.05), both in plasma and renal tissue. CONCLUSION: : Remote ischemic perconditioning was more effective reducing renal ischemia-reperfusion injury than administration of tramadol or association of both treatments.
PURPOSE: : To evaluate the effects of tramadol hydrochloride associated to remote ischemic perconditioning on oxidative stress. METHODS: : Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Tramadol group (T) treated with tramadol hydrochloride (40mg/kg); remote ischemic perconditioning + Tramadol group (Per+T) with both treatments. Oxidative stress was assessed after 24 hours of reperfusion. RESULTS: : Statistical differences were observed in MDA levels between I/R group with all groups (p<0.01), in addition there was difference between Tramadol with Sham, Per and Per+T groups (p<0.05), both in plasma and renal tissue. CONCLUSION: : Remote ischemic perconditioning was more effective reducing renal ischemia-reperfusion injury than administration of tramadol or association of both treatments.