Marcus Vinicius Henriques Brito1, Edson Yuzur Yasojima1, Sandro Percário2, Rubens Fernando Gonçalves Ribeiro3, Lainy Carollyne da Costa Cavalcante4, Andrew Moraes Monteiro5, Rodrigo Paracampo Couteiro5, Ivone Aline da Silva Rodrigues5, Hellen Aparecida Geyer Dos Santos5. 1. PhD, Full Professor, Department of Experimental Surgery, Universidade Estadual do Pará (UEPA), Belém-PA, Brazil. Conception, design and scientific content of the study, critical revision. 2. PhD, Full Professor, Department of Biological Sciences, UFPA, Belém-PA, Brazil. Conception, design and scientific content of the study, critical revision. 3. Master, Postgraduate Program in Surgery and Experimental Research, UEPA, Belém-PA, Brazil. Statistical analysis, manuscript preparation, English language. 4. Graduate student, School of Medicine, Centro Universitário do Pará (CESUPA), Belém-PA, Brazil. Technical procedures, interpretation of data, manuscript preparation. 5. Graduate student, School of Medicine, UEPA, Belém-PA, Brazil. Technical procedures, interpretation of data, manuscript preparation.
Abstract
PURPOSE: : To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in renal ischemia/reperfusion injury in rats. METHODS: : Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. After reperfusion, blood samples were collected for BUN and creatinine serum levels analyzes. TBARS were evaluated in plasma and renal tissue to assess oxidative stress. Kidney histopathological examination were performed. RESULTS: : Per+HSS group showed a lower degree of renal dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly reduced oxidative stress and histological damage. CONCLUSION: : Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute renal injury induced by ischemia/reperfusion.
PURPOSE: : To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in renal ischemia/reperfusion injury in rats. METHODS: : Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. After reperfusion, blood samples were collected for BUN and creatinine serum levels analyzes. TBARS were evaluated in plasma and renal tissue to assess oxidative stress. Kidney histopathological examination were performed. RESULTS: : Per+HSS group showed a lower degree of renal dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly reduced oxidative stress and histological damage. CONCLUSION: : Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute renal injury induced by ischemia/reperfusion.