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Literature DB >> 2840206

Effects of RNA secondary structure on alternative splicing of pre-mRNA: is folding limited to a region behind the transcribing RNA polymerase?

L P Eperon¹, I R Graham, A D Griffiths, I C Eperon.

Abstract

The use in vivo of an alternative 5' splice site sequestered within a short stem of potential RNA secondary structure is determined by the length of the loop. Above a threshold length of loop, the alternative site is used despite the potential structure. In contrast, the alternative site is used very little or not at all during splicing in vitro with all lengths of loop that we have tested. A model is proposed which suggests that pre-mRNA is free to fold only within a limited period after transcription.

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Year: 1988 PMID： 2840206 DOI： 10.1016/0092-8674(88)90202-4

Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582

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Cited

117 in total

1. Modulation of exon skipping by high-affinity hnRNP A1-binding sites and by intron elements that repress splice site utilization.

Authors: M Blanchette; B Chabot
Journal: EMBO J Date: 1999-04-01 Impact factor: 11.598

2. Multiple splicing defects in an intronic false exon.

Authors: H Sun; L A Chasin
Journal: Mol Cell Biol Date: 2000-09 Impact factor: 4.272

3. Multiple interdependent sequence elements control splicing of a fibroblast growth factor receptor 2 alternative exon.

Authors: F Del Gatto; A Plet; M C Gesnel; C Fort; R Breathnach
Journal: Mol Cell Biol Date: 1997-09 Impact factor: 4.272

4. An RNA polymerase pause site is associated with the immunoglobulin mus poly(A) site.

Authors: Martha L Peterson; Shannon Bertolino; Frankie Davis
Journal: Mol Cell Biol Date: 2002-08 Impact factor: 4.272

5. Assaying synthetic ribozymes in plants: high-level expression of a functional hammerhead structure fails to inhibit target gene activity in transiently transformed protoplasts.

Authors: L Mazzolini; M Axelos; N Lescure; P Yot
Journal: Plant Mol Biol Date: 1992-11 Impact factor: 4.076

Effects of RNA secondary structure on alternative splicing of pre-mRNA: is folding limited to a region behind the transcribing RNA polymerase?

1. Modulation of exon skipping by high-affinity hnRNP A1-binding sites and by intron elements that repress splice site utilization.

2. Multiple splicing defects in an intronic false exon.

3. Multiple interdependent sequence elements control splicing of a fibroblast growth factor receptor 2 alternative exon.

4. An RNA polymerase pause site is associated with the immunoglobulin mus poly(A) site.

5. Assaying synthetic ribozymes in plants: high-level expression of a functional hammerhead structure fails to inhibit target gene activity in transiently transformed protoplasts.

Review 6. Multiple links between transcription and splicing.

7. Alternative splicing of a human alpha-tropomyosin muscle-specific exon: identification of determining sequences.

8. TCERG1 regulates alternative splicing of the Bcl-x gene by modulating the rate of RNA polymerase II transcription.

9. Sequences involved in the control of adenovirus L1 alternative RNA splicing.

10. RNA secondary structure in mutually exclusive splicing.