Haci Murat Sagdik1, Fatma Ucar2, Mehmet Tetikoglu3, Serdar Aktas3, Fatih Ozcura3, Havva Kocak4, Salim Neselioglu5, Funda Eren5. 1. Department of Ophthalmology, Dumlupinar University School of Medicine, Kutahya, Turkey. dr.hsagdik@gmail.com. 2. Department of Clinical Biochemistry, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey. 3. Department of Ophthalmology, Dumlupinar University School of Medicine, Kutahya, Turkey. 4. Department of Clinical Biochemistry, Dumlupinar University School of Medicine, Kutahya, Turkey. 5. Department of Clinical Biochemistry, Ataturk Training and Research Hospital, Ankara, Turkey.
Abstract
PURPOSE: The aim of this study was to determine plasma thiol-disulphide homoeostasis in patients with age-related cataract (ARC) and compare the results of the patients with healthy subjects. Plasma malondialdehyde (MDA) levels and catalase (CAT) activity were also investigated. METHODS: The study included 53 cataract patients and 52 healthy volunteers. Native thiol-disulphide exchanges were determined using a novel and automated method. CAT activity was determined using the method described by Aebi, and MDA levels were calculated using the thiobarbituric acid method. RESULT: Native thiol and total thiol levels were significantly lower in the cataract patients compared with the controls (p < 0.001, p = 0.002, respectively). The disulphide levels of the cataract patients were significantly higher than the controls (p = 0.002). The ratios of disulphide/native thiol and disulphide/total thiol were statistically higher in the cataract patients compared with the control group (p < 0.001, p < 0.001, respectively). Furthermore, CAT activity was significantly lower in the cataract patient group compared with the control group (p < 0.001), and MDA levels were insignificantly higher in the patient group (p = 0.581). CONCLUSIONS: Our study showed that dynamic thiol-disulphide homoeostasis has shifted towards disulphide formation, as a result of thiol oxidation in ARC patients. The present study is the first to measure thiol-disulphide homoeostasis in ARC patients with a novel automated assay. This study supports the hypothesis that cataract is an oxidative disorder. Further studies are required in order to examine the relationship between oxidative stress and the development of cataract formation.
PURPOSE: The aim of this study was to determine plasma thiol-disulphide homoeostasis in patients with age-related cataract (ARC) and compare the results of the patients with healthy subjects. Plasma malondialdehyde (MDA) levels and catalase (CAT) activity were also investigated. METHODS: The study included 53 cataractpatients and 52 healthy volunteers. Native thiol-disulphide exchanges were determined using a novel and automated method. CAT activity was determined using the method described by Aebi, and MDA levels were calculated using the thiobarbituric acid method. RESULT: Native thiol and total thiol levels were significantly lower in the cataractpatients compared with the controls (p < 0.001, p = 0.002, respectively). The disulphide levels of the cataractpatients were significantly higher than the controls (p = 0.002). The ratios of disulphide/native thiol and disulphide/total thiol were statistically higher in the cataractpatients compared with the control group (p < 0.001, p < 0.001, respectively). Furthermore, CAT activity was significantly lower in the cataractpatient group compared with the control group (p < 0.001), and MDA levels were insignificantly higher in the patient group (p = 0.581). CONCLUSIONS: Our study showed that dynamic thiol-disulphide homoeostasis has shifted towards disulphide formation, as a result of thiol oxidation in ARCpatients. The present study is the first to measure thiol-disulphide homoeostasis in ARCpatients with a novel automated assay. This study supports the hypothesis that cataract is an oxidative disorder. Further studies are required in order to examine the relationship between oxidative stress and the development of cataract formation.
Authors: Silvia D Rodrigues; Giuseppe B Batista; Max Ingberman; Roberto Pecoits-Filho; Lia S Nakao Journal: Blood Purif Date: 2012-11-08 Impact factor: 2.614