Xiaoxiao Zhang1, Hongli Sun2, Qian Su3, Tianwei Lin4, Huiping Zhang3, Junli Zhang4, Shaokang Dang3, Zhongliang Zhu5. 1. Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 86-710069, PR China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi'an 86-710069, PR China. 2. Shaanxi Institute of Pediatric Diseases, Affiliated Children's Hospital of Xi'an Jiaotong University, Xi'an 86-710003, PR China. 3. Department of Neonatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 86-710061, PR China. 4. Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 86-710069, PR China. 5. Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 86-710069, PR China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi'an 86-710069, PR China. Electronic address: zlzhu@mail.xjtu.edu.cn.
Abstract
OBJECTIVE: The present study was performed to identify antidepressant-like activity of icariin in prenatally stressed male rats. METHODS: The effects of icariin on PRS-induced depression were examined using sucrose preference test (SPT) and forced swimming test (FST) in male offspring, and measuring protein and mRNA expressions of group I mGluRs receptors and EAAT2 via western blotting and quantitative real-time PCR assays. RESULTS: The results indicated that prenatal restraint stress (PRS) resulted in several behavioral anomalies. Treatment with icariin relieved the elevated protein and mRNA levels of group I mGluR receptors as well as the diminished protein and mRNA levels of EAAT2 in the PRS male offspring. CONCLUSIONS: Collectivity, the data support that icariin ameliorates PRS-induced depressive-like behavior via regulating expression of mGluR1, mGluR5 and EAAT2 in the hippocampus.
OBJECTIVE: The present study was performed to identify antidepressant-like activity of icariin in prenatally stressed male rats. METHODS: The effects of icariin on PRS-induced depression were examined using sucrose preference test (SPT) and forced swimming test (FST) in male offspring, and measuring protein and mRNA expressions of group I mGluRs receptors and EAAT2 via western blotting and quantitative real-time PCR assays. RESULTS: The results indicated that prenatal restraint stress (PRS) resulted in several behavioral anomalies. Treatment with icariin relieved the elevated protein and mRNA levels of group I mGluR receptors as well as the diminished protein and mRNA levels of EAAT2 in the PRS male offspring. CONCLUSIONS: Collectivity, the data support that icariin ameliorates PRS-induced depressive-like behavior via regulating expression of mGluR1, mGluR5 and EAAT2 in the hippocampus.
Authors: Caren J Blacker; Vincent Millischer; Lauren M Webb; Ada M C Ho; Martin Schalling; Mark A Frye; Marin Veldic Journal: Mol Neuropsychiatry Date: 2019-07-23
Authors: Nabil A Alhakamy; Usama A Fahmy; Shaimaa M Badr-Eldin; Osama A A Ahmed; Hani Z Asfour; Hibah M Aldawsari; Mardi M Algandaby; Basma G Eid; Ashraf B Abdel-Naim; Zuhier A Awan; Nabil K Alruwaili; Amir I Mohamed Journal: Pharmaceutics Date: 2020-04-11 Impact factor: 6.321