Literature DB >> 28395799

Generation of human iPSCs from urine derived cells of a non-affected control subject.

Muhammad Zeeshan Afzal1, Melanie Gartz1, Ekaterina A Klyachko2, Sadiya Sana Khan2, Sanjiv J Shah2, Sweta Gupta3, Amy D Shapiro3, Douglas E Vaughan2, Jennifer L Strande1.   

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.
Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

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Year:  2016        PMID： 28395799      PMCID： PMC5389122          DOI： 10.1016/j.scr.2016.12.008

Source DB:  PubMed          Journal:  Stem Cell Res        ISSN： 1873-5061            Impact factor:   2.020


  4 in total

1.  Brief report: complete deficiency of plasminogen-activator inhibitor type 1 due to a frame-shift mutation.

Authors:  W P Fay; A D Shapiro; J L Shih; R R Schleef; D Ginsburg
Journal:  N Engl J Med       Date:  1992-12-10       Impact factor: 91.245

2.  Human plasminogen activator inhibitor-1 (PAI-1) deficiency: characterization of a large kindred with a null mutation in the PAI-1 gene.

Authors:  W P Fay; A C Parker; L R Condrey; A D Shapiro
Journal:  Blood       Date:  1997-07-01       Impact factor: 22.113

3.  Generation of induced pluripotent stem cells from muscular dystrophy patients: efficient integration-free reprogramming of urine derived cells.

Authors:  Muhammad Z Afzal; Jennifer L Strande
Journal:  J Vis Exp       Date:  2015-01-28       Impact factor: 1.355

4.  Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

Authors:  Kazutoshi Takahashi; Koji Tanabe; Mari Ohnuki; Megumi Narita; Tomoko Ichisaka; Kiichiro Tomoda; Shinya Yamanaka
Journal:  Cell       Date:  2007-11-30       Impact factor: 41.582
  4 in total
  1 in total

Review 1.  Urine-Derived Stem Cells: The Present and the Future.

Authors:  Xiaoli Ji; Min Wang; Fang Chen; Junmei Zhou
Journal:  Stem Cells Int       Date:  2017-11-08       Impact factor: 5.443
  1 in total

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