Literature DB >> 28395797

Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts.

Marten Hansen1, Eszter Varga1, Tatjana Wüst1, Nelleke Brouwer2, Hugues Beauchemin3, Clemens Mellink4, Anne-Marie van der Kevie-Kersemaekers4, Tarik Möröy5, Bert van der Reijden2, Marieke von Lindern1, Emile van den Akker6.   

Abstract

Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers. The genomic integrity of iPSC line was shown by karyotyping. The MML-6838-Cl2 iPSC is, to our knowledge, the first to be generated from megakaryoblasts.
Copyright © 2016. Published by Elsevier B.V.

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Year:  2016        PMID： 28395797     DOI： 10.1016/j.scr.2016.12.004

Source DB:  PubMed          Journal:  Stem Cell Res        ISSN： 1873-5061            Impact factor:   2.020


  4 in total

1.  Large-scale in vitro production of red blood cells from human peripheral blood mononuclear cells.

Authors:  Steven Heshusius; Esther Heideveld; Patrick Burger; Marijke Thiel-Valkhof; Erica Sellink; Eszter Varga; Elina Ovchynnikova; Anna Visser; Joost H A Martens; Marieke von Lindern; Emile van den Akker
Journal:  Blood Adv       Date:  2019-11-12

2.  RUNX1 mutations enhance self-renewal and block granulocytic differentiation in human in vitro models and primary AMLs.

Authors:  Mylène Gerritsen; Guoqiang Yi; Esther Tijchon; Jorren Kuster; Jan Jacob Schuringa; Joost H A Martens; Edo Vellenga
Journal:  Blood Adv       Date:  2019-02-12

3.  Molecular mechanisms of bleeding disorderassociated GFI1BQ287* mutation and its affected pathways in megakaryocytes and platelets.

Authors:  Rinske van Oorschot; Marten Hansen; Johanna M Koornneef; Anna E Marneth; Saskia M Bergevoet; Maaike G J M van Bergen; Floris P J van Alphen; Carmen van der Zwaan; Joost H A Martens; Michiel Vermeulen; Pascal W T C Jansen; Marijke P A Baltissen; Britta A P Laros-van Gorkom; Hans Janssen; Joop H Jansen; Marieke von Lindern; Alexander B Meijer; Emile van den Akker; Bert A van der Reijden
Journal:  Haematologica       Date:  2019-01-17       Impact factor: 9.941

4.  The acute myeloid leukemia associated AML1-ETO fusion protein alters the transcriptome and cellular progression in a single-oncogene expressing in vitro induced pluripotent stem cell based granulocyte differentiation model.

Authors:  Esther Tijchon; Guoqiang Yi; Amit Mandoli; Jos G A Smits; Francesco Ferrari; Branco M H Heuts; Falco Wijnen; Bowon Kim; Eva M Janssen-Megens; Jan Jacob Schuringa; Joost H A Martens
Journal:  PLoS One       Date:  2019-12-23       Impact factor: 3.240
  4 in total

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