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Literature DB >> 2839464

Two lines of MDCK epithelial cells with different volume and ion responses to calcium ionophore A23187.

Abstract

Madin-Darby canine kidney (MDCK) cells lost volume when exposed to 50 microM calcium ionophore A23187. The ionic mechanism for the volume reduction differed between two types of cells. MDCK1 lost potassium, similar to cells undergoing a volume regulatory decrease in hypotonic medium. MDCK2 predominately lost sodium, as did cells with elevated cyclic AMP or when treated with amiloride. The effects of A23187 and dibutyryl-cyclic AMP on Na and K content and volume suggest that intracellular second messengers can trigger different mechanisms to reduce cell volume.

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Year: 1988 PMID： 2839464 DOI： 10.1007/BF02629095

Source DB: PubMed Journal: In Vitro Cell Dev Biol ISSN： 0883-8364

16 in total

Two lines of MDCK epithelial cells with different volume and ion responses to calcium ionophore A23187.

1. A COLORIMETRIC METHOD FOR DETERMINATION OF SERUM CHLORIDE.

2. LLC-PK1 cells: cloning of phenotypically stable subpopulations.

3. Variability of functional characteristics of MDCK cells.

4. Regulation of cell volume in separated renal tubules incubated in hypotonic medium.

5. Occluding junctions in cultured epithelial monolayers.

6. Effect of adenosine 3',5'-cyclic monophosphate on volume and cytoskeleton of MDCK cells.

7. Volume regulation by flounder red blood cells in anisotonic media.

8. Effect of hypotonic medium on K and Na content of proximal renal tubules.

9. Modulation of cyclic 3'5'-adenosine monophosphate in cultured renal (MDCK) cells by endogenous prostaglandins.

10. Cell volume regulation by Amphiuma red blood cells. The role of Ca+2 as a modulator of alkali metal/H+ exchange.

1. Differential effects of the calcium ionophore A23187 and the phorbol ester PMA on lymphocyte proliferation.

2. Glucocorticoid and polyamine involvement in zinc uptake by COMMA-1D mammary epithelial cells.