Chien-Neng Kuo1,2,3,4, Ching-Hsein Chen5, San-Ni Chen6,7, Jou-Cheng Huang1,2,3, Li-Ju Lai1,2,3, Chien-Hsiung Lai1,2,3, Chia-Hui Hung2,3,8, Chien-Hsing Lee9, Chung-Yi Chen10. 1. Department of Ophthalmology, Chang Gung Memorial Hospital, No. 6, W. Sec., Jiapu Rd, Puzi City, Chiayi County, 61363, Taiwan. 2. Chang Gung University College of Medicine, No. 259, Wenhua 1st Rd., Guishan Township, Taoyuan County, 33302, Taiwan. 3. Chang Gung University of Science and Technology, No. 2, W. Sec., Jiapu Rd., Puzi City, Chiayi County, 61363, Taiwan. 4. Department of Ophthalmology, Changhua Christian Hospital, Yun Lin Branch, No. 375, Shichang S. Rd., Xiluo Township, Yunlin County, 64866, Taiwan. 5. Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, No. 300 Syuefu Rd., Chiayi City, 60004, Taiwan. 6. Department of Ophthalmology, Changhua Christian Hospital, No. 135, Nanxiao St., Changhua City, Changhua County, 50006, Taiwan. 7. School of Medicine, Chung-Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung City, 40201, Taiwan. 8. Department of Dermatology, Chang Gung Memorial Hospital, No. 6, W. Sec., Jiapu Rd., Puzi City, Chiayi County, 61363, Taiwan. 9. Department of Pharmacology, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung City, 80708, Taiwan. chlee0818@gmail.com. 10. School of Medical and Health Sciences, Fooyin University, No. 151, Jinxue Rd., Daliao Dist., Kaohsiung City, 83102, Taiwan. xx377@fy.edu.tw.
Abstract
AIM: This study was to investigate the anti-angiogenic effect of hexahydrocurcumin (HHC) to evaluate gene (p-basic fibroblast growth factor (bFGF)-SAINT-18 & p-vascular endothelial growth factor (VEGF)-SAINT-18 complex)-induced corneal neovascularization (CorNV) in rats. METHODS: CorNV was induced in 24 eyes of 24 rats. Four groups (Group A: 0 μg, B: 0.01 μg, C: 0.1 μg, and D: 1 μg) of HHC were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at temporal side. The 1 μg of p-bFGF-SAINT-18 & p-VEGF-SAINT-18 complex were prepared and implanted into the rat corneal stroma 1.5 mm from the limbus at the same side. Inhibition of CorNV was observed and quantified from day 1 to day 60. bFGF and VEGF protein expression were analyzed by biomicroscopic examination, western blot analysis, and immunohistochemistry. RESULTS: Subconjunctival injection by 1 μg HHC successfully inhibited gene-induced CorNV in rats. bFGF and VEGF protein expression were reduced after 6 days. Meanwhile, the reduction of HLA-DR expression was detected. CONCLUSIONS: Our study showed that the HHC might provide an important anti-angiogenesis factor to inhibit CorNV development at the corneal experimental angiogenesis model.
AIM: This study was to investigate the anti-angiogenic effect of hexahydrocurcumin (HHC) to evaluate gene (p-basic fibroblast growth factor (bFGF)-SAINT-18 & p-vascular endothelial growth factor (VEGF)-SAINT-18 complex)-induced corneal neovascularization (CorNV) in rats. METHODS: CorNV was induced in 24 eyes of 24 rats. Four groups (Group A: 0 μg, B: 0.01 μg, C: 0.1 μg, and D: 1 μg) of HHC were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at temporal side. The 1 μg of p-bFGF-SAINT-18 & p-VEGF-SAINT-18 complex were prepared and implanted into the ratcorneal stroma 1.5 mm from the limbus at the same side. Inhibition of CorNV was observed and quantified from day 1 to day 60. bFGF and VEGF protein expression were analyzed by biomicroscopic examination, western blot analysis, and immunohistochemistry. RESULTS: Subconjunctival injection by 1 μg HHC successfully inhibited gene-induced CorNV in rats. bFGF and VEGF protein expression were reduced after 6 days. Meanwhile, the reduction of HLA-DR expression was detected. CONCLUSIONS: Our study showed that the HHC might provide an important anti-angiogenesis factor to inhibit CorNV development at the corneal experimental angiogenesis model.
Authors: U Benelli; G Bocci; R Danesi; A Lepri; N Bernardini; F Bianchi; M Lupetti; A Dolfi; A Campagni; C Agen; M Nardi; M Del Tacca Journal: Exp Eye Res Date: 1998-08 Impact factor: 3.467