| Literature DB >> 28391963 |
Uri Gophna1, Thorsten Allers2, Anita Marchfelder3.
Abstract
The majority of archaea encode CRISPR-Cas systems but only a few CRISPR-Cas-based genetic tools have been developed for organisms from this domain. Nayak and Metcalf have harnessed a bacterial Cas9 protein for genome editing in Methanosarcina acetivorans, enabling efficient gene deletion and replacement.Entities:
Keywords: CRISPR-Cas; Cas9; HDR; MMEJ; NHEJ; archaea
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Year: 2017 PMID： 28391963 DOI： 10.1016/j.tim.2017.03.009
Source DB: PubMed Journal: Trends Microbiol ISSN： 0966-842X Impact factor: 17.079