Aleksandra Piechota-Polanczyk1,2, Marcin Włodarczyk3,4, Aleksandra Sobolewska-Włodarczyk3,5, Mateusz Jonakowski3, Andrzej Pilarczyk3, Krystyna Stec-Michalska5, Maria Wiśniewska-Jarosińska5, Jakub Fichna3. 1. Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland. piechota.aleksandra@gmail.com. 2. Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland. piechota.aleksandra@gmail.com. 3. Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland. 4. Department of General and Colorectal Surgery, Faculty of Military Medicine, Medical University of Lodz, Lodz, Poland. 5. Department of Gastroenterology, Faculty of Military Medicine, Medical University of Lodz, Lodz, Poland.
Abstract
BACKGROUND: Cyclophilin A (CyPA) is an immunomodulatory protein, high expression of which correlates with poor outcome of patients with inflammatory diseases. However, its role in inflammatory bowel disease (IBD) has not been studied. AIM: This study analyzes the correlation between cyclophilin A, matrix metalloproteinase (MMP)-9, and tissue inhibitor of MMP (TIMP)/MMP-9 complexes in the inflamed and non-inflamed colon mucosa of UC and CD patients. METHODS: Serum and biopsy specimens from inflamed and non-inflamed colonic mucosa of 38 patients with IBD (19 with UC and 19 with CD) and 16 controls were included in our study. We measured serum and tissue level of CyPA, and tissue level of TNF-α, MMP-9, TIMP-1/MMP-9, and TIMP-2/MMP-9 using ELISA method. RESULTS: Our results indicated that serum, but not tissue CyPA is increased in UC, rather than in CD patients, compared to the control. The increase correlated with higher tissue concentration of MMP-9 and TNF-α, especially in the UC group. Moreover, we observed significantly higher level of TIMP-1/MMP-9 in UC and CD group, which overlapped with the change in MMP-9. There was no change in TIMP-2/MMP-9 in the analyzed groups. CONCLUSION: The current study suggests that serum CyPA may be an independent additional marker of IBD, especially of UC. Higher CyPA level may be followed by increased MMP-9 in those patients. However, further studies are necessary to verify the role of CyPA in IBD development.
BACKGROUND:Cyclophilin A (CyPA) is an immunomodulatory protein, high expression of which correlates with poor outcome of patients with inflammatory diseases. However, its role in inflammatory bowel disease (IBD) has not been studied. AIM: This study analyzes the correlation between cyclophilin A, matrix metalloproteinase (MMP)-9, and tissue inhibitor of MMP (TIMP)/MMP-9 complexes in the inflamed and non-inflamed colon mucosa of UC and CDpatients. METHODS: Serum and biopsy specimens from inflamed and non-inflamed colonic mucosa of 38 patients with IBD (19 with UC and 19 with CD) and 16 controls were included in our study. We measured serum and tissue level of CyPA, and tissue level of TNF-α, MMP-9, TIMP-1/MMP-9, and TIMP-2/MMP-9 using ELISA method. RESULTS: Our results indicated that serum, but not tissue CyPA is increased in UC, rather than in CDpatients, compared to the control. The increase correlated with higher tissue concentration of MMP-9 and TNF-α, especially in the UC group. Moreover, we observed significantly higher level of TIMP-1/MMP-9 in UC and CD group, which overlapped with the change in MMP-9. There was no change in TIMP-2/MMP-9 in the analyzed groups. CONCLUSION: The current study suggests that serum CyPA may be an independent additional marker of IBD, especially of UC. Higher CyPA level may be followed by increased MMP-9 in those patients. However, further studies are necessary to verify the role of CyPA in IBD development.
Authors: Q Gao; M J W Meijer; F J G M Kubben; C F M Sier; L Kruidenier; W van Duijn; M van den Berg; R A van Hogezand; C B H W Lamers; H W Verspaget Journal: Dig Liver Dis Date: 2005-08 Impact factor: 4.088
Authors: Karim Bahmed; Curtis Henry; Michael Holliday; Jasmina Redzic; Madalina Ciobanu; Fengli Zhang; Colin Weekes; Robert Sclafani; James Degregori; Elan Eisenmesser Journal: Cancer Cell Int Date: 2012-07-04 Impact factor: 5.722
Authors: Sarah R Calabro; Annette E Maczurek; Alison J Morgan; Thomas Tu; Victoria W Wen; Christine Yee; Auvro Mridha; Maggie Lee; William d'Avigdor; Stephen A Locarnini; Geoffrey W McCaughan; Fiona J Warner; Susan V McLennan; Nicholas A Shackel Journal: PLoS One Date: 2014-07-30 Impact factor: 3.240