David Péricat1, Anne Farina2, Emilie Agavnian-Couquiaud2, Christian Chabbert3, Brahim Tighilet4. 1. Aix Marseille Université, CNRS, UMR 7260, Laboratoire de Neurosciences Intégratives et Adaptatives - Equipe Physiopathologie et Thérapie des Désordres Vestibulaire, 3 Place Victor Hugo, 13331 Marseille Cedex 3, France. 2. IPC/CRCM Experimental Pathology (ICEP) Facility, Institut Paoli Calmettes, Marseille, France. 3. Aix Marseille Université, CNRS, UMR 7260, Laboratoire de Neurosciences Intégratives et Adaptatives - Equipe Physiopathologie et Thérapie des Désordres Vestibulaire, 3 Place Victor Hugo, 13331 Marseille Cedex 3, France. Electronic address: christian.chabbert@univ-amu.fr. 4. Aix Marseille Université, CNRS, UMR 7260, Laboratoire de Neurosciences Intégratives et Adaptatives - Equipe Physiopathologie et Thérapie des Désordres Vestibulaire, 3 Place Victor Hugo, 13331 Marseille Cedex 3, France. Electronic address: brahim.tighilet@univ-amu.fr.
Abstract
BACKGROUND: Both basic and applied studies on the pathophysiology of vestibular disorders are currently impaired by the lack of animal models of controlled vestibular damages. NEW METHODS: In the present study, we describe the procedure to achieve a surgical unilateral vestibular neurectomy (UVN) in the rat and evaluate its functional consequences. This procedure is suitable for reproducing a unilateral, sudden and definitive vestibular areflexia. RESULTS: Proper induction of a UVN induces a severe vestibular syndrome, which mimics vestibular disorders encountered in humans. This model is also used clinically in the surgical treatment of pharmacological intractable Meniere's disease. Comparison with existing methods unilateral vestibular neurectomy has been essentially used in other species such as cats, monkeys and humans. The current study describes this technique in rats. CONCLUSION: This experimental model is particularly adapted to study the restoration kinetics of vestibular function after removal of peripheral inputs. It is also suitable for determining the neurochemical and molecular mechanisms underlying central compensation processes, as well as to check for the efficacy of drugs with potent antivertigo properties. Finally, UVN is an acknowledged model of postlesional plasticity involving original processes such as reactive neurogenesis in the vestibular nuclei.
BACKGROUND: Both basic and applied studies on the pathophysiology of vestibular disorders are currently impaired by the lack of animal models of controlled vestibular damages. NEW METHODS: In the present study, we describe the procedure to achieve a surgical unilateral vestibular neurectomy (UVN) in the rat and evaluate its functional consequences. This procedure is suitable for reproducing a unilateral, sudden and definitive vestibular areflexia. RESULTS: Proper induction of a UVN induces a severe vestibular syndrome, which mimics vestibular disorders encountered in humans. This model is also used clinically in the surgical treatment of pharmacological intractable Meniere's disease. Comparison with existing methods unilateral vestibular neurectomy has been essentially used in other species such as cats, monkeys and humans. The current study describes this technique in rats. CONCLUSION: This experimental model is particularly adapted to study the restoration kinetics of vestibular function after removal of peripheral inputs. It is also suitable for determining the neurochemical and molecular mechanisms underlying central compensation processes, as well as to check for the efficacy of drugs with potent antivertigo properties. Finally, UVN is an acknowledged model of postlesional plasticity involving original processes such as reactive neurogenesis in the vestibular nuclei.
Authors: Mun Young Chang; Moo Kyun Park; So Hyeon Park; Myung-Whan Suh; Jun Ho Lee; Seung Ha Oh Journal: J Vis Exp Date: 2018-05-19 Impact factor: 1.355
Authors: Raphaelle Cassel; Pierrick Bordiga; Julie Carcaud; François Simon; Mathieu Beraneck; Anne Le Gall; Anne Benoit; Valentine Bouet; Bruno Philoxene; Stéphane Besnard; Isabelle Watabe; David Pericat; Charlotte Hautefort; Axel Assie; Alain Tonetto; Jonas Dyhrfjeld-Johnsen; Jordi Llorens; Brahim Tighilet; Christian Chabbert Journal: Dis Model Mech Date: 2019-07-15 Impact factor: 5.758
Authors: Emna Marouane; Guillaume Rastoldo; Nada El Mahmoudi; David Péricat; Christian Chabbert; Vincent Artzner; Brahim Tighilet Journal: Front Neurol Date: 2020-05-29 Impact factor: 4.003