| Literature DB >> 28389942 |
Lewis D B Evans1, Paul M Bergen1, Owain J Bryant1, Gillian M Fraser2.
Abstract
During assembly of the bacterial flagellum, structural subunits synthesized inside the cell must be exported across the cytoplasmic membrane before they can crystallize into the nascent flagellar structure. This export process is facilitated by a specialized Flagellar Type III Secretion System (fT3SS) located at the base of each flagellum. Here, we describe three methods-isothermal titration calorimetry, photo-crosslinking using unnatural amino acids, and a subunit capture assay-used to investigate the interactions of flagellar structural subunits with the membrane export machinery component FlhB.Entities:
Keywords: Capture assay; Flagella; Flagellar type III secretion system (fT3SS); Isothermal titration calorimetry (ITC); Protein export; Protein-protein interactions; Salmonella; p-benzoyl-L-phenylalanine (pBpa) photo-crosslinking
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Year: 2017 PMID： 28389942 DOI： 10.1007/978-1-4939-6927-2_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745