Pretreated algal bloom as a substantial nutrient source for microalgae cultivation for biodiesel production.

In the present investigation, toxic algal bloom, a copious and low-cost nutrient source was deployed for cultivating Chlorella pyrenoidosa. Various pre-treatment methods using combinations of acid/alkali and autoclave/microwave were tested for preparing hydrolysates and compared with minimal media (BG-11). Acid autoclave treatment resulted in maximum carbon, nitrogen and phosphorous content which substantially boosted the growth of the microalgal cells (4.36g/L) as compared to rest of the media. The microalga grown in this media also showed enhanced lipid content (43.2%) and lipid productivity (188mg/L/d) as compared to BG-11 (19.42mg/L/d). The biochemical composition showed 1.6-fold declines in protein while 1.27 folds in carbohydrate content as compared to BG-11. The fatty acid profile revealed the presence of C14-C22 with increased amount of monounsaturated fatty acids as compared to BG-11. The results obtained showed that algal bloom can be used as a potential nutrient source for microalgae.