Haifeng Yu1, Ping Duan2, Haibo Zhu1, Dapang Rao1. 1. Department of Urology, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou 325000, Zhejiang, China. 2. Department of Obstetric and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou 325000, Zhejiang, China.
Abstract
OBJECTIVES: Increasing evidence has suggested that microRNA (miRNA) dysregulation may contribute to tumor progression and metastasis. However, the role of miR-613 in bladder cancer was still unknown. MATERIALS AND METHODS: qRT-PCR and Western blotting were performed to detect the expression of miR-613 and its direct target gene. CCK-8 analysis, qRT-PCR and cell invasion were performed to measure the cell function. RESULTS: We demonstrated that the expression of miR-613 was downregulated in the bladder cancer cell lines. In addition, miR-613 expression was downregulated in the bladder cancer tissues compared to the adjacent normal tissues. Out of 35 bladder cancer tissues, miR-613 was downregulated in 27 cases compared to the adjacent tissues. Ectopic expression of miR-613 suppressed the bladder cancer cell proliferation and invasion. Moreover, miR-613 overexpression enhanced the expression of epithelial biomarker, Ecadherin, and suppressed the expression of mesenchymal biomarker, Vimentin, Snail and N-cadherin. Furthermore, we identified the Sphingosine kinase 1 (SphK1) as the direct target gene of miR-613 in the bladder cancer cell. Restoration of Sphk1 partially rescued miR-613-inhibited bladder cancer cell proliferation, invasion and EMT. CONCLUSIONS: These data suggested that miR-613 acted a tumor suppressive role in bladder cancer through targeting SphK1 in bladder.
OBJECTIVES: Increasing evidence has suggested that microRNA (miRNA) dysregulation may contribute to tumor progression and metastasis. However, the role of miR-613 in bladder cancer was still unknown. MATERIALS AND METHODS: qRT-PCR and Western blotting were performed to detect the expression of miR-613 and its direct target gene. CCK-8 analysis, qRT-PCR and cell invasion were performed to measure the cell function. RESULTS: We demonstrated that the expression of miR-613 was downregulated in the bladder cancer cell lines. In addition, miR-613 expression was downregulated in the bladder cancer tissues compared to the adjacent normal tissues. Out of 35 bladder cancer tissues, miR-613 was downregulated in 27 cases compared to the adjacent tissues. Ectopic expression of miR-613 suppressed the bladder cancer cell proliferation and invasion. Moreover, miR-613 overexpression enhanced the expression of epithelial biomarker, Ecadherin, and suppressed the expression of mesenchymal biomarker, Vimentin, Snail and N-cadherin. Furthermore, we identified the Sphingosine kinase 1 (SphK1) as the direct target gene of miR-613 in the bladder cancer cell. Restoration of Sphk1 partially rescued miR-613-inhibited bladder cancer cell proliferation, invasion and EMT. CONCLUSIONS: These data suggested that miR-613 acted a tumor suppressive role in bladder cancer through targeting SphK1 in bladder.
Authors: Ruth L Vinall; Alexandra Z Ripoll; Sisi Wang; Chong-Xian Pan; Ralph W deVere White Journal: Int J Cancer Date: 2011-12-02 Impact factor: 7.396
Authors: Stella Koutros; Debra T Silverman; Michael Cr Alavanja; Gabriella Andreotti; Catherine C Lerro; Sonya Heltshe; Charles F Lynch; Dale P Sandler; Aaron Blair; Laura E Beane Freeman Journal: Int J Epidemiol Date: 2015-09-27 Impact factor: 7.196