| Literature DB >> 2838498 |
F Denis1, G Leonard, A Sangare, G Gershy-Damet, J L Rey, B Soro, D Schmidt, M Mounier, M Verdier, A Baillou.
Abstract
The efficacies of nine enzyme-linked immunosorbent assays (EIA) for antibody to human immunodeficiency virus type 1 (HIV-1) and one EIA for antibody to HIV-2 in detecting antibody to HIV-2 were studied. The competitive EIAs for antibody to HIV-1 were less sensitive than the indirect EIAs. The overall prevalence of positive results was between 28 and 51% with the competitive EIAs and between 70 and 93% with the indirect EIAs. Most of the EIAs were less sensitive in detecting antibody to HIV-2 in sera from people with acquired immunodeficiency syndrome-like diseases than in sera from symptomless individuals. The results indicate that there is a high degree of cross-reactivity between HIV-1 and HIV-2 by EIA, indicating that serotype specificity must be determined by Western blot (immunoblot) with both sets of viral antigens. The results are relevant for discussing public health strategies, especially the screening of blood donors; competitive EIAs for antibody to HIV-1 are not sensitive enough to be used in areas where HIV-2 is prevalent (West Africa).Entities:
Keywords: Acquired Immunodeficiency Syndrome; Africa; Africa South Of The Sahara; Antibodies; Biology; Clinical Research; Developing Countries; Diseases; Examinations And Diagnoses; French Speaking Africa; Hematologic Tests; Hiv Infections; Immunity; Immunologic Factors; Ivory Coast; Laboratory Examinations And Diagnoses; Laboratory Procedures; Physiology; Research Methodology; Viral Diseases; Western Africa
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Year: 1988 PMID： 2838498 PMCID： PMC266504 DOI： 10.1128/jcm.26.5.1000-1004.1988
Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948