Literature DB >> 2838497

Analytical strategy for determination of active site sequences in aminoacyl-tRNA synthetases.

C Beauvallet1, C Hountondji, J M Schmitter.   

Abstract

Affinity labelling with radioactive, periodate-oxidized tRNA has been used to investigate the structures of tRNA-binding sites in Escherichia coli aminoacyl-tRNA synthetases. Labelled peptides were isolated by means of a combination of techniques involving chymotryptic digestion of the enzyme, gel filtration, ribonuclease digestion of tRNA, chromatography on a TSK 2000 column and reversed-phase chromatography. An isocratic phenylthiohydantoin identification system has been interfaced to a sequencer, allowing the characterization of modified lysine residues by means of both chromatographic retention and liquid scintillation counting.

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Year:  1988        PMID： 2838497     DOI： 10.1016/s0021-9673(00)90266-8

Source DB:  PubMed          Journal:  J Chromatogr


  7 in total

1.  Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli.

Authors:  J M Guillon; Y Mechulam; J M Schmitter; S Blanquet; G Fayat
Journal:  J Bacteriol       Date:  1992-07       Impact factor: 3.490

2.  Catabolism of bis(5'-nucleosidyl) tetraphosphates in Saccharomyces cerevisiae.

Authors:  P Plateau; M Fromant; J M Schmitter; S Blanquet
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

3.  Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species.

Authors:  F Lévêque; P Plateau; P Dessen; S Blanquet
Journal:  Nucleic Acids Res       Date:  1990-01-25       Impact factor: 16.971

4.  Properties of the lysyl-tRNA synthetase gene and product from the extreme thermophile Thermus thermophilus.

Authors:  J Chen; A Brevet; M Lapadat-Tapolsky; S Blanquet; P Plateau
Journal:  J Bacteriol       Date:  1994-05       Impact factor: 3.490

5.  Methionyl-tRNA synthetase from Bacillus stearothermophilus: structural and functional identities with the Escherichia coli enzyme.

Authors:  Y Mechulam; E Schmitt; M Panvert; J M Schmitter; M Lapadat-Tapolsky; T Meinnel; P Dessen; S Blanquet; G Fayat
Journal:  Nucleic Acids Res       Date:  1991-07-11       Impact factor: 16.971

6.  Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid.

Authors:  P H Hirel; M J Schmitter; P Dessen; G Fayat; S Blanquet
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

7.  Primary Structure Revision and Active Site Mapping of E. Coli Isoleucyl-tRNA Synthetase by Means of Maldi Mass Spectrometry.

Authors:  Soria Baouz; Jean-Marie Schmitter; Lila Chenoune; Christian Beauvallet; Sylvain Blanquet; Anne Woisard; Codjo Hountondji
Journal:  Open Biochem J       Date:  2009-03-06
  7 in total

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