Deepa Rajesh1, Chaitra Chowdappa2, Rajesh Gurumurthy3, A V Moideen Kutty4, Sharath Balakrishna5. 1. Research Assistant, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka, India . 2. Postgraduate Student, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka, India . 3. Assistant Professor, Department of Dermatology, Sri Devaraj Urs Medical College , Kolar, Karnataka, India . 4. Professor, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka, India . 5. Assistant Professor, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka, India .
Abstract
INTRODUCTION: Tumour necrosis factor-alpha (TNFα) gene -308G/A polymorphism (rs1800629) are associated with psoriasis in several populations worldwide. Presently, there is no literature on the status of this polymorphism in the South Indian population. AIM: To determine the profile of TNFα -308G/A polymorphism among psoriatic patients. MATERIALS AND METHODS: This case-control study involved 74 patients with Psoriasis Vulgaris (PsV) and 74 age and gender matched healthy individuals. Patients were recruited from the Department of Dermatology of R.L. Jalappa Hospital and Research Center, Tamaka, Kolar, Karnataka, India, from March 2014 to March 2016. TNFα -308G/A polymorphism was genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. RESULTS: The frequency of TNFα -308A allele 7.4% among psoriatic and 8.8% among non-psoriatic individuals. The difference was not statistically significant (p=0.82). CONCLUSION: Our results indicate that TNFα gene -308G/A polymorphism is not a significant marker for the risk of developing PsV among South Indian (Karnataka) psoriatic patients.
INTRODUCTION:Tumour necrosis factor-alpha (TNFα) gene -308G/A polymorphism (rs1800629) are associated with psoriasis in several populations worldwide. Presently, there is no literature on the status of this polymorphism in the South Indian population. AIM: To determine the profile of TNFα -308G/A polymorphism among psoriaticpatients. MATERIALS AND METHODS: This case-control study involved 74 patients with Psoriasis Vulgaris (PsV) and 74 age and gender matched healthy individuals. Patients were recruited from the Department of Dermatology of R.L. Jalappa Hospital and Research Center, Tamaka, Kolar, Karnataka, India, from March 2014 to March 2016. TNFα -308G/A polymorphism was genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. RESULTS: The frequency of TNFα -308A allele 7.4% among psoriatic and 8.8% among non-psoriatic individuals. The difference was not statistically significant (p=0.82). CONCLUSION: Our results indicate that TNFα gene -308G/A polymorphism is not a significant marker for the risk of developing PsV among South Indian (Karnataka) psoriaticpatients.
Authors: Catherine T Jordan; Li Cao; Elisha D O Roberson; Shenghui Duan; Cynthia A Helms; Rajan P Nair; Kristina Callis Duffin; Philip E Stuart; David Goldgar; Genki Hayashi; Emily H Olfson; Bing-Jian Feng; Clive R Pullinger; John P Kane; Carol A Wise; Raphaela Goldbach-Mansky; Michelle A Lowes; Lynette Peddle; Vinod Chandran; Wilson Liao; Proton Rahman; Gerald G Krueger; Dafna Gladman; James T Elder; Alan Menter; Anne M Bowcock Journal: Am J Hum Genet Date: 2012-04-19 Impact factor: 11.025
Authors: Kati Asumalahti; Mahreen Ameen; Sari Suomela; Eva Hagforsen; Gerd Michaëlsson; Julie Evans; Margo Munro; Colin Veal; Michael Allen; Joyce Leman; A David Burden; Brian Kirby; Maureen Connolly; Christopher E M Griffiths; Richard C Trembath; Juha Kere; Ulpu Saarialho-Kere; Jonathan N W N Barker Journal: J Invest Dermatol Date: 2003-04 Impact factor: 8.551