Theerawut Thammakun1, Wongsa Laohasiriwong2, Ratthaphol Kraiklang3, Nittaya Saengprajak4. 1. Faculty, Department of Public Health, Khon Kaen University and Research and Training Center for Enhancing Quality of Life of Working Age People, Khon Kaen University , Khon Kaen, Thailand . 2. Associate Professor, Department of Public Health, Khon Kaen University and Board Committee of Research and Training Centre for Enhancing Quality of Life of Working Age People (REQW), Khon Kaen University , Khon Kaen, Thailand . 3. Lecturer, Department of Public Health, Khon Kaen University and Research Group on Prevention and Control of Diabetes in the Northeast , Khon Kaen, Thailand . 4. Lecturer, Department of Public Health, Kalasin University , Kalasin, Thailand .
Abstract
INTRODUCTION: Resistin gene (RETN) polymorphisms in humans may have a role in the pathogenesis of Type 2 Diabetes Mellitus (T2DM) and insulin resistance. There is still lack of evidence on association between +62 G>A polymorphism in the RETN and T2DM among Thais. AIM: To determine the effect of polymorphisms at +62 G>A of RETN on Thai T2DM. MATERIALS AND METHODS: This matched case control study was conducted with a total of 360 samples from all regions of Thailand (180 Thai new T2DM cases and 180 non-T2DM Thais for control) were enrolled. The RETN +62G>A polymorphism were detected using the Polymerase Chain Reaction (PCR) method. Conditional logistic regression was performed to test the association between +62 G>A polymorphism and T2DM. RESULTS: Among 360 samples that were enrolled, only 350 samples completed molecular analysis. It was found that GA+AA genotype frequencies in T2DM cases was higher than control by 16% (95% CI: 6.0%, 27.0%, p-value=0.002). After adjustments for possible confounders, multivariable analyses by conditional logistic regression showed that the RETN+62 G>A polymorphism was statistically associated with Thai T2DM (ORadjusted =1.84, 95% CI: 1.03, 3.31, p-value=0.04). Other factors such as; low educational attainment (ORadjusted=3.87, 95%CI: 1.60, 9.36), hypertension (ORadjusted=3.07, 95%CI: 1.56, 6.04), had both obese father and mother (ORadjusted=1.94, 95%CI: 1.06, 3.56) and triglyceride≥150 (ORadjusted=2.18, 95% CI: 1.18, 4.02) were statistically associated with Thai T2DM (p-value<0.05). While regular consumption of glutinous rice was found to be a protective factor (ORadjusted=0.29, 95%CI: 0.13, 0.64). CONCLUSION: These findings suggest that RETN polymorphism at position +62 G>A may increase the susceptibility to T2DM in Thais.
INTRODUCTION:Resistin gene (RETN) polymorphisms in humans may have a role in the pathogenesis of Type 2 Diabetes Mellitus (T2DM) and insulin resistance. There is still lack of evidence on association between +62 G>A polymorphism in the RETN and T2DM among Thais. AIM: To determine the effect of polymorphisms at +62 G>A of RETN on Thai T2DM. MATERIALS AND METHODS: This matched case control study was conducted with a total of 360 samples from all regions of Thailand (180 Thai new T2DM cases and 180 non-T2DM Thais for control) were enrolled. The RETN +62G>A polymorphism were detected using the Polymerase Chain Reaction (PCR) method. Conditional logistic regression was performed to test the association between +62 G>A polymorphism and T2DM. RESULTS: Among 360 samples that were enrolled, only 350 samples completed molecular analysis. It was found that GA+AA genotype frequencies in T2DM cases was higher than control by 16% (95% CI: 6.0%, 27.0%, p-value=0.002). After adjustments for possible confounders, multivariable analyses by conditional logistic regression showed that the RETN+62 G>A polymorphism was statistically associated with Thai T2DM (ORadjusted =1.84, 95% CI: 1.03, 3.31, p-value=0.04). Other factors such as; low educational attainment (ORadjusted=3.87, 95%CI: 1.60, 9.36), hypertension (ORadjusted=3.07, 95%CI: 1.56, 6.04), had both obese father and mother (ORadjusted=1.94, 95%CI: 1.06, 3.56) and triglyceride≥150 (ORadjusted=2.18, 95% CI: 1.18, 4.02) were statistically associated with Thai T2DM (p-value<0.05). While regular consumption of glutinous rice was found to be a protective factor (ORadjusted=0.29, 95%CI: 0.13, 0.64). CONCLUSION: These findings suggest that RETN polymorphism at position +62 G>A may increase the susceptibility to T2DM in Thais.