Purpose: The purpose of this study was characterization of adult murine neuroretina in organ culture to investigate its suitability for use in preclinical therapeutic applications. In retinal disorders, neurodegeneration of mature retinal cells takes place. Therefore, neonatal retina cultures are not adequate for therapeutic applications, such as genome editing, as the retina is still developing with cells dividing and differentiating into highly specialized cell types such as photoreceptors. Methods: Retinal explants were prepared from 3-month-old wild-type C57Bl6 mice and evaluated after 1 to 10 days in culture by immunohistochemistry or quantitative (q)PCR. Histologic modifications of the neuroretina were evaluated with TUNEL assay and immunohistochemical markers for neurons, glia cells, and apoptosis. Results: During the first week, disruption and truncation of outer segments were detectable. Unspecific Müller cell reaction was detected from 4 days in culture. Sprouting of individual rod bipolar cell dendrites into the outer nuclear layer (ONL) was visible during all explant stages. During the second week in culture, cell death in the ONL became more prominent. Conclusions: Adult organotypic retinal culture was successful for at least 10 days with characteristic alterations of the morphology during this period. This characterization forms the basis to establish retinal explants for gene therapeutic applications as an intermediate step between cell culture and experiments on adult animals, thus reducing the load of animal experimentation.
Purpose: The purpose of this study was characterization of adult murine neuroretina in organ culture to investigate its suitability for use in preclinical therapeutic applications. In retinal disorders, neurodegeneration of mature retinal cells takes place. Therefore, neonatal retina cultures are not adequate for therapeutic applications, such as genome editing, as the retina is still developing with cells dividing and differentiating into highly specialized cell types such as photoreceptors. Methods: Retinal explants were prepared from 3-month-old wild-type C57Bl6 mice and evaluated after 1 to 10 days in culture by immunohistochemistry or quantitative (q)PCR. Histologic modifications of the neuroretina were evaluated with TUNEL assay and immunohistochemical markers for neurons, glia cells, and apoptosis. Results: During the first week, disruption and truncation of outer segments were detectable. Unspecific Müller cell reaction was detected from 4 days in culture. Sprouting of individual rod bipolar cell dendrites into the outer nuclear layer (ONL) was visible during all explant stages. During the second week in culture, cell death in the ONL became more prominent. Conclusions: Adult organotypic retinal culture was successful for at least 10 days with characteristic alterations of the morphology during this period. This characterization forms the basis to establish retinal explants for gene therapeutic applications as an intermediate step between cell culture and experiments on adult animals, thus reducing the load of animal experimentation.
Authors: Yu Zhu; Bowen Cao; Arianna Tolone; Jie Yan; Gustav Christensen; Blanca Arango-Gonzalez; Marius Ueffing; François Paquet-Durand Journal: Front Neurosci Date: 2022-07-07 Impact factor: 5.152
Authors: Sizar Kamar; Marcus H C Howlett; Jan Klooster; Wim de Graaff; Tamás Csikós; Martijn J W E Rabelink; Rob C Hoeben; Maarten Kamermans Journal: Int J Mol Sci Date: 2020-01-14 Impact factor: 5.923
Authors: Jesús David Urbano-Gámez; Lourdes Valdés-Sánchez; Carmen Aracil; Berta de la Cerda; Francisco Perdigones; Álvaro Plaza Reyes; Francisco J Díaz-Corrales; Isabel Relimpio López; José Manuel Quero Journal: Micromachines (Basel) Date: 2021-11-29 Impact factor: 2.891