| Literature DB >> 2838395 |
T I Tikchonenko1, S E Glushakova, O S Kislina, N A Grodnitskaya, A A Manykin, B S Naroditsky.
Abstract
High-molecular-weight viral DNAs have been packed into proteoliposomes prepared by reverse-phase evaporation followed by phospholipid membrane targeting by influenza virus glycoprotein bound to hydrophobic 'anchors'. DNA has been encapsulated in the form of spermine condensates--toroidal structures sized approx. 0.1 micron, resistant to ultrasound. The efficiency of entrapping into liposomes reached 30% for condensed DNA of Mr up to 3 X 10(7). Specific infectivity of simian virus 40 DNA and simian adenovirus DNA packed into such proteoliposomes was 50- to 100-fold higher than that shown by free DNA preparations under Ca.phosphate-precipitation conditions.Entities:
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Year: 1988 PMID： 2838395 DOI： 10.1016/0378-1119(88)90535-5
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688