Isolation of four rat creatine kinase genes and identification of multiple potential promoter sequences within the rat brain creatine kinase promoter region.

Rat genes encoding muscle creatine kinase (gene ckm) and brain creatine kinase (gene ckb) have been cloned. The two genes have an identical intron-exon distribution, although introns of the ckb gene are much smaller than those for ckm. The complete nucleotide sequence has been determined for the ckb gene. 5'-Flanking sequences have been compared for both ckm and ckb. The ckb gene has a structurally complex 5'-flanking region with multiple TATA and CAAT sequences suggesting that the gene may contain overlapping promoter elements. Examination of ckb RNA from brain tissue provides no evidence for the existence of more than one set of transcriptional start points. The ckm and ckb genes show little homology in the 5'-flanking regions, although we observed some sequence conservation in the regions immediately surrounding the CAAT and TATA sequences. The rat genome also contains two other creatine kinase-like sequences which may represent processed pseudogenes or mitochondrial creatine kinase.