Literature DB >> 2838384

Chemical synthesis, cloning and expression in mammalian cells of a gene coding for human tissue-type plasminogen activator.

L D Bell1, J C Smith, R Derbyshire, M Finlay, I Johnson, R Gilbert, P Slocombe, E Cook, H Richards, P Clissold.   

Abstract

A 1610-bp DNA duplex coding for human tissue-type plasminogen activator has been chemically synthesized using the phosphoramidite procedure, adapted for a custom-built gene synthesizer. The synthesizer, which was designed for both simplicity and speed, permits the rapid construction of relatively large genes and compares favorably in speed with alternative cDNA isolation procedures. The plasminogen activator gene has been expressed in mammalian cells and shown to produce authentic protein by an immuno-activity assay.

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Year:  1988        PMID： 2838384     DOI： 10.1016/0378-1119(88)90521-5

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  4 in total

1.  Synthesis of a gene for human serum albumin and its expression in Saccharomyces cerevisiae.

Authors:  M Kálmán; I Cserpán; G Bajszár; A Dobi; E Horváth; C Pázmán; A Simoncsits
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

2.  Error rates in oligodeoxynucleotides synthesized by the H-phosphonate method.

Authors:  M Vasser; P G Ng; P Jhurani; N Bischofberger
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

3.  Rapid generation of DNA fragments by PCR amplification of crude, synthetic oligonucleotides.

Authors:  R W Barnett; H Erfle
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

4.  Synthetic gene for the hepatitis C virus nucleocapsid protein.

Authors:  Y E Khudyakov; H A Fields; M O Favorov; N S Khudyakova; M T Bonafonte; B Holloway
Journal:  Nucleic Acids Res       Date:  1993-06-11       Impact factor: 16.971
  4 in total

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