Literature DB >> 28382319

Ca2+ Measurements in Mammalian Cells with Aequorin-based Probes.

Anna Tosatto1, Rosario Rizzuto1, Cristina Mammucari1.   

Abstract

Aequorin is a Ca2+ sensitive photoprotein suitable to measure intracellular Ca2+ transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate measurement of Ca2+ uptake and release of different intracellular organelles. Here, we describe how to use this probe to measure cytosolic Ca2+ levels and mitochondrial Ca2+ uptake in mammalian cells.

Entities:  

Keywords:  Aequorin; Ca2+; Luminescence; Probes

Year:  2017        PMID： 28382319      PMCID： PMC5378298          DOI： 10.21769/BioProtoc.2155

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  7 in total

1.  The crystal structure of the photoprotein aequorin at 2.3 A resolution.

Authors:  J F Head; S Inouye; K Teranishi; O Shimomura
Journal:  Nature       Date:  2000-05-18       Impact factor: 49.962

Review 2.  Mitochondrial protein import.

Authors:  F U Hartl; N Pfanner; D W Nicholson; W Neupert
Journal:  Biochim Biophys Acta       Date:  1989-01-18

3.  Use of calcium-regulated photoproteins as intracellular Ca2+ indicators.

Authors:  J R Blinks
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

4.  Using targeted variants of aequorin to measure Ca2+ levels in intracellular organelles.

Authors:  Veronica Granatiero; Maria Patron; Anna Tosatto; Giulia Merli; Rosario Rizzuto
Journal:  Cold Spring Harb Protoc       Date:  2014-01-01

5.  The use of aequorin and its variants for Ca2+ measurements.

Authors:  Veronica Granatiero; Maria Patron; Anna Tosatto; Giulia Merli; Rosario Rizzuto
Journal:  Cold Spring Harb Protoc       Date:  2014-01-01

6.  Calcium-sensitive photoproteins.

Authors:  Marisa Brini
Journal:  Methods       Date:  2008-10-10       Impact factor: 3.608

7.  Transfected aequorin in the measurement of cytosolic Ca2+ concentration ([Ca2+]c). A critical evaluation.

Authors:  M Brini; R Marsault; C Bastianutto; J Alvarez; T Pozzan; R Rizzuto
Journal:  J Biol Chem       Date:  1995-04-28       Impact factor: 5.157
  7 in total
  1 in total

1.  Identification and functional validation of FDA-approved positive and negative modulators of the mitochondrial calcium uniporter.

Authors:  Agnese De Mario; Anna Tosatto; Julia Marie Hill; Janos Kriston-Vizi; Robin Ketteler; Denis Vecellio Reane; Gino Cortopassi; Gyorgy Szabadkai; Rosario Rizzuto; Cristina Mammucari
Journal:  Cell Rep       Date:  2021-06-22       Impact factor: 9.423
  1 in total

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