Literature DB >> 2837921

Nonradioactive labeling of synthetic oligonucleotide probes with terminal deoxynucleotidyl transferase.

A Kumar1, P Tchen, F Roullet, J Cohen.   

Abstract

Synthetic oligonucleotides were tailed at the 3' end using terminal deoxynucleotidyl transferase. Nucleotide triphosphates with free primary amines at the end of side chains were compared for their tailing efficiency and/or detection sensitivity, using biotin-11-dUTP as a reference. Free primary amines were tagged with activated biotin or fluorescein isothiocyanate. The probes were then detected with either streptavidin-alkaline phosphatase complex or anti-fluorescein antibodies and alkaline phosphatase-conjugated secondary antibodies. Tailing conditions were optimized and the probes were tested for detection of Escherichia coli ST1a enterotoxin DNA and rotavirus RNA.

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Year:  1988        PMID： 2837921     DOI： 10.1016/0003-2697(88)90299-0

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  15 in total

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5.  Chemiluminescent detection of DNA: application for DNA sequencing and hybridization.

Authors:  S Beck; T O'Keeffe; J M Coull; H Köster
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6.  Immunocytochemistry of 5-bromo-2'-deoxyuridine labelled oligonucleotide probes. A novel technique for in situ hybridization.

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7.  Enzymatic synthesis and modification of high molecular weight DNA using terminal deoxynucleotidyl transferase.

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9.  In situ hybridization of semithin Epon sections with BrdU labelled oligonucleotide probes.

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Journal:  Histochemistry       Date:  1990

10.  Detection of Mycoplasma hyopneumoniae by using rRNA-oligodeoxynucleotide hybridization.

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