L Polo1, R Mañes-Lázaro1, I Olmeda1, L E Cruz-Pio1, Á Medina2, S Ferrer1, I Pardo1. 1. ENOLAB, Estructura de Recerca Interdisciplinar (ERI) BioTecMed, University of Valencia, Dr. Moliner 50, Burjassot, Valencia, 46100, Spain. 2. Department of Microbiology and Ecology, University of Valencia, Dr. Moliner 50, Burjassot, Valencia, 46100, Spain.
Abstract
AIMS: To determine the effect of three different freezing temperatures on post-freeze-drying survival rates of wine yeasts and lactic acid bacteria (LAB). To know if a similar freeze-drying protocol can be used for both micro-organisms. METHODS AND RESULTS: Cells from liquid culture media were recovered and concentrated in appropriate lyoprotectants. Aliquots of each strain were frozen at -20, -80 and -196°C before vacuum drying. Viable cell counts were done before freezing and after freeze-drying. Survival rates were calculated. Freezing temperatures differently affected yeast and bacteria survival. The highest survival rates were obtained at -20 and -80°C for yeasts, but at -196°C for LAB. Major differences in survival rates were recorded among freeze-dried yeasts, but were less drastic for LAB. Yeasts Pichia membranifaciens, Starmerella bacillaris and Metschnikowia pulcherrima, and LAB Lactobacillus paracasei, Pediococcus parvulus and Lactobacillus mali, were the most tolerant species to freeze-drying, regardless of freezing temperature. CONCLUSIONS: Yeast and LAB survival rates differed for each tested freezing temperature. For yeasts, -20°C ensured the highest post-freeze-drying viability and -196°C for LAB. SIGNIFICANCE AND IMPACT OF THE STUDY: Freezing temperature to freeze-dry cells is a crucial factor for ensuring good wine yeast and LAB survival. These results are important for appropriately preserving micro-organisms and for improving starter production processes.
AIMS: To determine the effect of three different freezing temperatures on post-freeze-drying survival rates of wine yeasts and lactic acid bacteria (LAB). To know if a similar freeze-drying protocol can be used for both micro-organisms. METHODS AND RESULTS: Cells from liquid culture media were recovered and concentrated in appropriate lyoprotectants. Aliquots of each strain were frozen at -20, -80 and -196°C before vacuum drying. Viable cell counts were done before freezing and after freeze-drying. Survival rates were calculated. Freezing temperatures differently affected yeast and bacteria survival. The highest survival rates were obtained at -20 and -80°C for yeasts, but at -196°C for LAB. Major differences in survival rates were recorded among freeze-dried yeasts, but were less drastic for LAB. YeastsPichia membranifaciens, Starmerella bacillaris and Metschnikowia pulcherrima, and LAB Lactobacillus paracasei, Pediococcus parvulus and Lactobacillus mali, were the most tolerant species to freeze-drying, regardless of freezing temperature. CONCLUSIONS:Yeast and LAB survival rates differed for each tested freezing temperature. For yeasts, -20°C ensured the highest post-freeze-drying viability and -196°C for LAB. SIGNIFICANCE AND IMPACT OF THE STUDY: Freezing temperature to freeze-dry cells is a crucial factor for ensuring good wine yeast and LAB survival. These results are important for appropriately preserving micro-organisms and for improving starter production processes.
Authors: Nádia N Batista; Cíntia L Ramos; Josiane F Pires; Silvino I Moreira; Eduardo Alves; Disney R Dias; Rosane F Schwan Journal: Food Sci Nutr Date: 2019-04-23 Impact factor: 2.863
Authors: D Panisello Yagüe; J Mihaljevic; M Mbegbu; C V Wood; C Hepp; S Kyman; H Hornstra; R Trotter; E Cope; T Pearson Journal: J Appl Microbiol Date: 2021-02-05 Impact factor: 3.772
Authors: Sandeep Dhall; Malathi Sathyamoorthy; Jin-Qiang Kuang; Tyler Hoffman; Matthew Moorman; Anne Lerch; Vimal Jacob; Steven Michael Sinclair; Alla Danilkovitch Journal: PLoS One Date: 2018-10-02 Impact factor: 3.240