| Literature DB >> 2837516 |
M C Vissers1, S A Jester, J C Fantone.
Abstract
We have developed a rapid and simple method for isolating human peripheral blood monocytes in suspension. The procedure combines two separation media and involves isolation of the mononuclear cells by centrifugation through Ficoll-Hypaque followed by purification of the monocytes using Sepracell-MN, a colloidal silica-based medium. The final cell population contained approximately 90% monocytes with good functional ability. The contaminating cells were lymphocytes. Viability was always greater than or equal to 99% with 90% recovery of the monocytes from the mononuclear cells.Entities:
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Year: 1988 PMID： 2837516 DOI： 10.1016/0022-1759(88)90104-4
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303