Literature DB >> 28373491

Isolation of High-Molecular-Weight DNA Using Organic Solvents.

Michael R Green, Joseph Sambrook.   

Abstract

Perhaps the most basic of all procedures in molecular cloning is the purification of nucleic acids. The key step, the removal of proteins, can often be performed simply by extracting aqueous solutions of nucleic acids with phenol:chloroform and chloroform.
© 2017 Cold Spring Harbor Laboratory Press.

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Year:  2017        PMID： 28373491     DOI： 10.1101/pdb.prot093450

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  50 in total

1.  Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes.

Authors:  Günther Schmalzing; Fritz Markwardt
Journal:  Methods Mol Biol       Date:  2022

2.  Human mitochondrial DNA is extensively methylated in a non-CpG context.

Authors:  Vibha Patil; Cyrille Cuenin; Felicia Chung; Jesus R Rodriguez Aguilera; Nora Fernandez-Jimenez; Irati Romero-Garmendia; Jose Ramon Bilbao; Vincent Cahais; Joseph Rothwell; Zdenko Herceg
Journal:  Nucleic Acids Res       Date:  2019-11-04       Impact factor: 16.971

3.  Detecting circRNA in purified spliceosomal P complex.

Authors:  Shasha Shi; Xueni Li; Rui Zhao
Journal:  Methods       Date:  2021-02-10       Impact factor: 3.608

4.  Phenotype Expansion for Atypical Gaucher Disease Due to Homozygous Missense PSAP Variant in a Large Consanguineous Pakistani Family.

Authors:  Khurram Liaqat; Shabir Hussain; Anushree Acharya; Abdul Nasir; Thashi Bharadwaj; Muhammad Ansar; Sulman Basit; Isabelle Schrauwen; Wasim Ahmad; Suzanne M Leal
Journal:  Genes (Basel)       Date:  2022-04-09       Impact factor: 4.141

5.  The Acetyltransferase RibT From Bacillus subtilis Affects in vivo Dynamics of the Multimeric Heavy Riboflavin Synthase Complex.

Authors:  Daniel Andreas Orlando Rotter; Christoph Heger; Christian Kühm; Nina Schmidt; Antje Schäfer; Thomas Heimerl; Matthias Mack; Peter L Graumann
Journal:  Front Microbiol       Date:  2022-04-14       Impact factor: 5.640

6.  Metagenome Sequencing of Prokaryotic Microbiota from Two Hypersaline Soils of the Odiel Salt Marshes in Huelva, Southwestern Spain.

Authors:  Blanca Vera-Gargallo; Laura Navarro-Sampedro; Modesto Carballo; Antonio Ventosa
Journal:  Genome Announc       Date:  2018-03-01

7.  Molecular characterization of canine parvovirus variants (CPV-2a, CPV-2b, and CPV-2c) based on the VP2 gene in affected domestic dogs in Ecuador.

Authors:  David De la Torre; Eulalia Mafla; Byron Puga; Linda Erazo; Claudete Astolfi-Ferreira; Antonio Piantino Ferreira
Journal:  Vet World       Date:  2018-04-16

8.  Metagenomic Insights into the Phylogenetic and Metabolic Diversity of the Prokaryotic Community Dwelling in Hypersaline Soils from the Odiel Saltmarshes (SW Spain).

Authors:  Blanca Vera-Gargallo; Antonio Ventosa
Journal:  Genes (Basel)       Date:  2018-03-08       Impact factor: 4.096

9.  Genome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution.

Authors:  Ling Zhu; Muhammad A Shahid; John Markham; Glenn F Browning; Amir H Noormohammadi; Marc S Marenda
Journal:  BMC Genomics       Date:  2018-02-02       Impact factor: 3.969

10.  Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Infantis Strain SPE101, Isolated from a Chronic Human Infection.

Authors:  Andrés Iriarte; Joaquín Giner-Lamia; Claudia Silva; Laura Betancor; Lizeth Astocondor; Juan J Cestero; Theresa Ochoa; Coralith García; José L Puente; José A Chabalgoity; Francisco García-Del Portillo
Journal:  Genome Announc       Date:  2017-07-20
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