Jéssica Silva1, Regina Arantes-Rodrigues2,3, Rosário Pinto-Leite3,4, Ana I Faustino-Rocha2,5, Lio Fidalgo-Gonçalves6, Lúcio Santos4,7,8, Paula A Oliveira9,2. 1. Department of Veterinary Sciences, Hospital Center of Trás-os-Montes and Alto Douro, Vila Real, Portugal. 2. Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Hospital Center of Trás-os-Montes and Alto Douro, Vila Real, Portugal. 3. Cytogenetic Laboratory, Hospital Center of Trás-os-Montes and Alto Douro, Vila Real, Portugal. 4. Experimental Pathology and Therapeutics Group, Portuguese Institute of Oncology, Porto, Portugal. 5. Faculty of Veterinary Medicine, Lusophone University of Humanities and Technologies, Lisbon, Portugal. 6. Institute for Systems and Computer Engineering, Technology and Science (INESC-TEC), University of Trás-os-Montes and Alto Douro, Vila Real, Portugal. 7. Health School, University Fernando Pessoa, Porto, Portugal. 8. Medical Oncology Department, Portuguese Institute of Oncology, Porto, Portugal. 9. Department of Veterinary Sciences, Hospital Center of Trás-os-Montes and Alto Douro, Vila Real, Portugal pamo@utad.pt.
Abstract
BACKGROUND/AIM: This study aimed to evaluate the in vitro efficacy of carboplatin and piroxicam, both in isolation and combined, against T24 and 5637 human urinary bladder cancer cell lines. MATERIALS AND METHODS: Cell viability, drug interaction, cell morphology, cell proliferation, apoptosis and autophagy were analyzed after 72 h of drug exposure. Statistical analysis was performed and values of p<0.05 were considered statistically significant. RESULTS: Drug exposure in combination led to a significant reduction of cell viability comparatively to single-drug exposure. These combinations resulted in a synergistic interaction in the T24 (combination index for 50% effect (CI50)=0.65) and 5637 (CI50=0.17) cell lines. Notable increase of morphological alterations, a marked decrease of Ki-67 expression, a considerable enhancement of autophagic vacuoles and a minimal effect on apoptosis was observed in both cell lines treated with combined drugs. CONCLUSION: Data showed that in vitro combination of carboplatin and piroxicam produced a more potent antiproliferative effect when compared to single drugs. Copyright
BACKGROUND/AIM: This study aimed to evaluate the in vitro efficacy of carboplatin and piroxicam, both in isolation and combined, against T24 and 5637 human urinary bladder cancer cell lines. MATERIALS AND METHODS: Cell viability, drug interaction, cell morphology, cell proliferation, apoptosis and autophagy were analyzed after 72 h of drug exposure. Statistical analysis was performed and values of p<0.05 were considered statistically significant. RESULTS: Drug exposure in combination led to a significant reduction of cell viability comparatively to single-drug exposure. These combinations resulted in a synergistic interaction in the T24 (combination index for 50% effect (CI50)=0.65) and 5637 (CI50=0.17) cell lines. Notable increase of morphological alterations, a marked decrease of Ki-67 expression, a considerable enhancement of autophagic vacuoles and a minimal effect on apoptosis was observed in both cell lines treated with combined drugs. CONCLUSION: Data showed that in vitro combination of carboplatin and piroxicam produced a more potent antiproliferative effect when compared to single drugs. Copyright
Authors: Smita Kumari; Sudhanshu Sharma; Dia Advani; Akanksha Khosla; Pravir Kumar; Rashmi K Ambasta Journal: Environ Sci Pollut Res Int Date: 2021-10-05 Impact factor: 5.190
Authors: Dirk Rades; Liesa Dziggel; Lisa Manig; Stefan Janssen; Mai Trong Khoa; Vuong Ngoc Duong; Vu Huu Khiem; Steven E Schild Journal: In Vivo Date: 2018 May-Jun Impact factor: 2.155
Authors: Dorota Lachowicz; Agnieszka Kaczyńska; Anna Bodzon-Kulakowska; Anna Karewicz; Roma Wirecka; Michał Szuwarzyński; Szczepan Zapotoczny Journal: Int J Mol Sci Date: 2020-12-18 Impact factor: 5.923