Literature DB >> 28373197

PCR-Dipstick Chromatography for Differential Detection of Carbapenemase Genes Directly in Stool Specimens.

Rathina Kumar Shanmugakani1,2,3, Yukihiro Akeda4,2,3, Norihisa Yamamoto1,2,3, Noriko Sakamoto3, Hideharu Hagiya1,2,3, Hisao Yoshida1,2,3, Dan Takeuchi3, Yo Sugawara3, Takuya Kodera5, Mitsuo Kawase5,6, Warawut Laolerd7, Narong Chaihongsa7, Pitak Santanirand7, Yoshikazu Ishii8, Shigeyuki Hamada3, Kazunori Tomono1,2.   

Abstract

A PCR-dipstick chromatography technique was designed and evaluated for differential identification of blaNDM, blaKPC, blaIMP, and blaOXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.
Copyright © 2017 American Society for Microbiology.

Keywords:  Enterobacteriaceae; carbapenemase; molecular diagnostics

Mesh：

Substances：

Year:  2017        PMID： 28373197      PMCID： PMC5444138          DOI： 10.1128/AAC.00067-17

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  12 in total

1.  Evaluation of clinical outcomes in patients with bloodstream infections due to Gram-negative bacteria according to carbapenem MIC stratification.

Authors:  John S Esterly; Jamie Wagner; Milena M McLaughlin; Michael J Postelnick; Chao Qi; Marc H Scheetz
Journal:  Antimicrob Agents Chemother       Date:  2012-07-09       Impact factor: 5.191

Review 2.  Review of established and innovative detection methods for carbapenemase-producing Gram-negative bacteria.

Authors:  J Osei Sekyere; U Govinden; S Y Essack
Journal:  J Appl Microbiol       Date:  2015-09-24       Impact factor: 3.772

3.  PCR-dipstick DNA chromatography for profiling of a subgroup of caries-associated bacterial species in plaque from healthy coronal surfaces and periodontal pockets.

Authors:  Lingyang Tian; Takuichi Sato; Kousuke Niwa; Mitsuo Kawase; Gen Mayanagi; Jumpei Washio; Nobuhiro Takahashi
Journal:  Biomed Res       Date:  2016       Impact factor: 1.203

4.  Suitability of Carbapenem Inactivation Method (CIM) for Detection of IMP Metallo-β-Lactamase-Producing Enterobacteriaceae.

Authors:  Kyoichi Saito; Ryuichi Nakano; Yuki Suzuki; Akiyo Nakano; Yoshihiko Ogawa; Shinsuke Yonekawa; Shiro Endo; Fumiko Mizuno; Kei Kasahara; Keiichi Mikasa; Mitsuo Kaku; Hisakazu Yano
Journal:  J Clin Microbiol       Date:  2017-01-18       Impact factor: 5.948

5.  A rapid and enhanced DNA detection method for crop cultivar discrimination.

Authors:  Yuki Monden; Kazuto Takasaki; Satoshi Futo; Kousuke Niwa; Mitsuo Kawase; Hiroto Akitake; Makoto Tahara
Journal:  J Biotechnol       Date:  2014-06-19       Impact factor: 3.307

6.  Continuing Challenges for the Clinical Laboratory for Detection of Carbapenem-Resistant Enterobacteriaceae.

Authors:  Romney M Humphries; James A McKinnell
Journal:  J Clin Microbiol       Date:  2015-10-14       Impact factor: 5.948

Review 7.  Carbapenem-Resistant Acinetobacter baumannii and Enterobacteriaceae in South and Southeast Asia.

Authors:  Li-Yang Hsu; Anucha Apisarnthanarak; Erum Khan; Nuntra Suwantarat; Abdul Ghafur; Paul Anantharajah Tambyah
Journal:  Clin Microbiol Rev       Date:  2016-10-19       Impact factor: 26.132

Review 8.  The Problem of Carbapenemase-Producing-Carbapenem-Resistant-Enterobacteriaceae Detection.

Authors:  Joseph D Lutgring; Brandi M Limbago
Journal:  J Clin Microbiol       Date:  2016-01-06       Impact factor: 5.948

Review 9.  Strategies for identification of carbapenemase-producing Enterobacteriaceae.

Authors:  Patrice Nordmann; Laurent Poirel
Journal:  J Antimicrob Chemother       Date:  2012-10-26       Impact factor: 5.790

10.  Development of selective medium for IMP-type carbapenemase-producing Enterobacteriaceae in stool specimens.

Authors:  Norihisa Yamamoto; Ryuji Kawahara; Yukihiro Akeda; Rathina Kumar Shanmugakani; Hisao Yoshida; Hideharu Hagiya; Naohiro Hara; Isao Nishi; Satomi Yukawa; Rumiko Asada; Yumi Sasaki; Kazuhiro Maeda; Noriko Sakamoto; Shigeyuki Hamada; Kazunori Tomono
Journal:  BMC Infect Dis       Date:  2017-03-24       Impact factor: 3.090
View more
  4 in total

1.  Current state of the art in rapid diagnostics for antimicrobial resistance.

Authors:  Rathina Kumar Shanmugakani; Balaji Srinivasan; Marshall J Glesby; Lars F Westblade; Washington B Cárdenas; Tony Raj; David Erickson; Saurabh Mehta
Journal:  Lab Chip       Date:  2020-07-09       Impact factor: 6.799

2.  Dissemination of carbapenemase-producing Enterobacteriaceae harbouring blaNDM or blaIMI in local market foods of Yangon, Myanmar.

Authors:  Yo Sugawara; Hideharu Hagiya; Yukihiro Akeda; Mya Mya Aye; Hpoo Pwint Myo Win; Noriko Sakamoto; Rathina Kumar Shanmugakani; Dan Takeuchi; Isao Nishi; Akiko Ueda; Moh Moh Htun; Kazunori Tomono; Shigeyuki Hamada
Journal:  Sci Rep       Date:  2019-10-08       Impact factor: 4.379

3.  Establishment of a dual-wavelength spectrophotometric method for analysing and detecting carbapenemase-producing Enterobacteriaceae.

Authors:  Dan Takeuchi; Yukihiro Akeda; Yo Sugawara; Noriko Sakamoto; Norihisa Yamamoto; Rathina Kumar Shanmugakani; Takuma Ishihara; Ayumi Shintani; Kazunori Tomono; Shigeyuki Hamada
Journal:  Sci Rep       Date:  2018-10-24       Impact factor: 4.379

4.  Design and Evaluation of Multiplex One-Step Reverse Transcription PCR-Dipstick Chromatography Method for the Analysis of Seven Respiratory Pathogens.

Authors:  Li Luo; Qianming Chen; Sheng Qin; Qiang Luo; Zhenjie Liu; Qiong Li; Shuilan Zheng; Xianzhang Huang; Peifeng Ke; Xiangsheng Yang; Hui Xiao; Ning Xu
Journal:  Curr Microbiol       Date:  2021-08-02       Impact factor: 2.188
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.