Clara I Bayarri-Lara1, Diego de Miguel Pérez2,3, Antonio Cueto Ladrón de Guevara1, Antonio Rodriguez Fernández4, Jose L Puche2,5, Abel Sánchez-Palencia Ramos1, Javier Ruiz Zafra1, Carlos F Giraldo Ospina1, Miguel Delgado-Rodríguez6, Manuela Expósito Ruiz7, Maria José Moyano Rodriguez1, Jose A Lorente2,3, Maria José Serrano2,6. 1. Department of Thoracic Surgery, Virgen de las Nieves University Hospital, Granada, Spain. 2. Liquid Biopsy and Metastasis Research Group, GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government-PTS Granada, Granada, Spain. 3. Laboratory of Genetic Identification, Legal Medicine and Toxicology Department, Faculty of Medicine, University of Granada, Granada, Spain. 4. Department of Nuclear Medicine, Virgen de las Nieves University Hospital, Granada, Spain. 5. Division of Integral Oncology, University Hospital of Granada, Granada, Spain. 6. Division of Preventive Medicine and Public Health, University of Jaén, Jaén, Spain. 7. Division for Methodological Assessment, Alejandro Otero Biomedical Research Fundation (FIBAO), Granada, Spain.
Abstract
OBJECTIVES: More than 20% of lung cancer patients develop a recurrence, even after curative resection. We hypothesized that relapse may arise from the dissemination of circulating tumour cells (CTCs). This study evaluates the significance of CTC detection as regards the recurrence of non-small-cell lung cancer (NSCLC) in surgically resected patients. Secondly, we investigated the association between CTCs and the uptake of 18 F-fluorodeoxyglucose (FDG) by the primary tumour on a positron emission tomographic (PET) scan. METHODS: In this single-centre prospective study, blood samples for analysis of CTCs were obtained from 102 patients with Stage I-IIIA NSCLC both before (CTC1) and 1 month after (CTC2) radical resection. CTCs were isolated using immunomagnetic techniques. The presence of CTCs was correlated with the maximum standardized uptake value (SUVmax) measured on preoperative FDG PET/computed tomographic scans. Recurrence free survival (RFS) analysis was performed. RESULTS: CTCs were detected in 39.2% of patients before and in 27.5% 1 month after the operation. The presence of CTCs after the operation was significantly correlated with SUVmax on PET scans, pathological stage and surgical approach. Only SUVmax was an independent predictor for the presence of CTC2 on multivariate analysis. Postoperative CTCs were significantly correlated with a shorter RFS ( P = 0.005). In multivariate analysis, the presence of CTC2 was associated with RFS, independent of disease staging. CONCLUSIONS: Detection of CTCs 1 month after radical resection might be a useful marker to predict early recurrence in Stage I-III NSCLC. The SUVmax value of the primary tumour on preoperative PET scans was associated with the presence of CTC 1 month after the operation.
OBJECTIVES: More than 20% of lung cancerpatients develop a recurrence, even after curative resection. We hypothesized that relapse may arise from the dissemination of circulating tumour cells (CTCs). This study evaluates the significance of CTC detection as regards the recurrence of non-small-cell lung cancer (NSCLC) in surgically resected patients. Secondly, we investigated the association between CTCs and the uptake of 18 F-fluorodeoxyglucose (FDG) by the primary tumour on a positron emission tomographic (PET) scan. METHODS: In this single-centre prospective study, blood samples for analysis of CTCs were obtained from 102 patients with Stage I-IIIA NSCLC both before (CTC1) and 1 month after (CTC2) radical resection. CTCs were isolated using immunomagnetic techniques. The presence of CTCs was correlated with the maximum standardized uptake value (SUVmax) measured on preoperative FDG PET/computed tomographic scans. Recurrence free survival (RFS) analysis was performed. RESULTS:CTCs were detected in 39.2% of patients before and in 27.5% 1 month after the operation. The presence of CTCs after the operation was significantly correlated with SUVmax on PET scans, pathological stage and surgical approach. Only SUVmax was an independent predictor for the presence of CTC2 on multivariate analysis. Postoperative CTCs were significantly correlated with a shorter RFS ( P = 0.005). In multivariate analysis, the presence of CTC2 was associated with RFS, independent of disease staging. CONCLUSIONS: Detection of CTCs 1 month after radical resection might be a useful marker to predict early recurrence in Stage I-III NSCLC. The SUVmax value of the primary tumour on preoperative PET scans was associated with the presence of CTC 1 month after the operation.
Authors: Diego M Avella; Yariswamy Manjunath; Amolak Singh; Chelsea B Deroche; Eric T Kimchi; Kevin F Staveley-O'Carroll; Jonathan B Mitchem; Eric Kwon; Guangfu Li; Jussuf T Kaifi Journal: Transl Lung Cancer Res Date: 2020-06
Authors: Diego de Miguel-Pérez; Clara Isabel Bayarri-Lara; Francisco Gabriel Ortega; Alessandro Russo; María José Moyano Rodriguez; Maria Jesus Alvarez-Cubero; Elizabeth Maza Serrano; José Antonio Lorente; Christian Rolfo; María José Serrano Journal: Cancers (Basel) Date: 2019-11-07 Impact factor: 6.639