Kian Eftekhari1,2, M Reza Vagefi3, Vivian Lee4, James Z Hui5, Menglong Zhu6, Kimberly Dine2, Richard L Anderson7, Brigitte Koeberlein8, Reas Sulaimankutty2, Kenneth S Shindler2,4. 1. Division of Ophthalmic Plastic and Reconstructive Surgery, Department of Ophthalmology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina. 2. FM Kirby Center for Molecular Ophthalmology, Stellar-Chance Laboratories, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania. 3. Division of Oculoplastic, Orbital and Reconstructive Surgery, Department of Ophthalmology, University of California, San Francisco, San Francisco, California. 4. Department of Ophthalmology, Scheie Eye Institute. 5. Department of Bioengineering, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania. 6. General Robotics, Automation, Sensing & Perception (GRASP) Laboratory, University of Pennsylvania, Philadelphia, Pennsylvania. 7. Oculoplastic Surgery, Inc., Salt Lake City, Utah. 8. Harrison Department of Surgical Research, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, U.S.A.
Abstract
PURPOSE: Recent publications have reported the adverse effects of prostaglandin analogues on the periocular tissues. These medications may cause periorbital lipodystrophy, enophthalmos, and deepening of the superior sulcus deformity. While these effects may have adverse consequences for some patients, the atrophy of the periorbital fat may have a useful role in diseases that lead to orbital and periorbital fat hypertrophy such as thyroid eye disease. In this pilot study, the authors investigated the effects of retrobulbar bimatoprost injection on the intraocular pressure and orbital fat in a rat animal model. METHODS: Three rats were sedated and intraocular pressure was measured. A 0.1 ml aliquot of bimatoprost was injected into the right orbit of all rats. In the left orbit, 0.1 ml of phosphate-buffered saline was injected as a control. Three weeks later, all rats were sedated and intraocular pressure was measured before euthanizing. Routine histologic staining was performed and thin sections through the intraconal orbital fat were obtained. Density of intraconal adipocytes was measured and adipocyte heterogeneity was determined using a computer image analysis algorithm. RESULTS: The specimens injected with bimatoprost demonstrated atrophy of orbital fat with significantly increased adipocyte density (p = 0.009) and heterogeneity (p = 0.008) when compared with control. Intraocular pressure was not significantly decreased at 3 weeks after injection of retrobulbar bimatoprost. CONCLUSIONS: In this pilot study, orbital injection of bimatoprost demonstrated atrophy of intraconal adipocytes when compared with control orbits injected with saline. The orbits injected with bimatoprost were noted to have smaller, more heterogeneous adipocytes that were densely packed in the intraconal space. The study limitations include the small sample size, which limited the ability for us to make conclusions about the effect on intraocular pressure. Nevertheless, the findings presented suggest that retrobulbar bimatoprost may present a nonsurgical alternative to induce atrophy of the orbital fat without inducing inflammation or hypotony.
PURPOSE: Recent publications have reported the adverse effects of prostaglandin analogues on the periocular tissues. These medications may cause periorbital lipodystrophy, enophthalmos, and deepening of the superior sulcus deformity. While these effects may have adverse consequences for some patients, the atrophy of the periorbital fat may have a useful role in diseases that lead to orbital and periorbital fathypertrophy such as thyroid eye disease. In this pilot study, the authors investigated the effects of retrobulbar bimatoprost injection on the intraocular pressure and orbital fat in a rat animal model. METHODS: Three rats were sedated and intraocular pressure was measured. A 0.1 ml aliquot of bimatoprost was injected into the right orbit of all rats. In the left orbit, 0.1 ml of phosphate-buffered saline was injected as a control. Three weeks later, all rats were sedated and intraocular pressure was measured before euthanizing. Routine histologic staining was performed and thin sections through the intraconal orbital fat were obtained. Density of intraconal adipocytes was measured and adipocyte heterogeneity was determined using a computer image analysis algorithm. RESULTS: The specimens injected with bimatoprost demonstrated atrophy of orbital fat with significantly increased adipocyte density (p = 0.009) and heterogeneity (p = 0.008) when compared with control. Intraocular pressure was not significantly decreased at 3 weeks after injection of retrobulbar bimatoprost. CONCLUSIONS: In this pilot study, orbital injection of bimatoprost demonstrated atrophy of intraconal adipocytes when compared with control orbits injected with saline. The orbits injected with bimatoprost were noted to have smaller, more heterogeneous adipocytes that were densely packed in the intraconal space. The study limitations include the small sample size, which limited the ability for us to make conclusions about the effect on intraocular pressure. Nevertheless, the findings presented suggest that retrobulbar bimatoprost may present a nonsurgical alternative to induce atrophy of the orbital fat without inducing inflammation or hypotony.
Authors: Cristoforo Silvestri; Andrea Martella; Neil J Poloso; Fabiana Piscitelli; Raffaele Capasso; Angelo Izzo; David F Woodward; Vincenzo Di Marzo Journal: J Biol Chem Date: 2013-06-25 Impact factor: 5.157
Authors: Catherine J Choi; Wensi Tao; Ravi Doddapaneni; Zenith Acosta-Torres; Nathan W Blessing; Bradford W Lee; Daniel Pelaez; Sara T Wester Journal: Invest Ophthalmol Vis Sci Date: 2018-12-03 Impact factor: 4.799