Afshin Shirkani1, Atena Mansouri2, Mohammad Reza Abbaszadegan3, Reza Faridhosseini4, Farahzad Jabbari Azad4, Mehran Gholamin5. 1. School of medicine allergy and clinical immunology department, Blusher University of medical science, Blusher Iran. 2. Student Research Committee , Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. 3. Medical Genetics Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran. 4. Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 5. Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.; Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract
BACKGROUND: The development of allergic rhinitis (AR) is caused by the interaction between genetic predisposition and environmental factors. In this study, the association between GATA3 single nucleotide polymorphisms and AR in an Iranian population was identified. METHODS: This case-control study was performed on 86 patients with AR and 86 healthy subjects. This study aimed to evaluate a potential association between two GATA3 SNPs, rs1269486 and rs2229360, and AR. Blood samples were collected and DNA was extracted for the evaluation of these SNPs by RFLP-PCR. RESULTS: A statistically-significant association was found between rs1269486 and AR (P<0.001). The frequencies of the A and GA genotypes were less in patients than in controls. The frequencies of the G allele and the GG genotype were greater in patients than in controls (P < 0.001). CONCLUSIONS: SNP rs1269486 of GATA3 was associated with AR and sensitivity to aeroallergens in our population. Because of the significance of this gene in AR, studying the association between GATA3 polymorphisms and AR is recommended for other populations.
BACKGROUND: The development of allergic rhinitis (AR) is caused by the interaction between genetic predisposition and environmental factors. In this study, the association between GATA3 single nucleotide polymorphisms and AR in an Iranian population was identified. METHODS: This case-control study was performed on 86 patients with AR and 86 healthy subjects. This study aimed to evaluate a potential association between two GATA3 SNPs, rs1269486 and rs2229360, and AR. Blood samples were collected and DNA was extracted for the evaluation of these SNPs by RFLP-PCR. RESULTS: A statistically-significant association was found between rs1269486 and AR (P<0.001). The frequencies of the A and GA genotypes were less in patients than in controls. The frequencies of the G allele and the GG genotype were greater in patients than in controls (P < 0.001). CONCLUSIONS: SNP rs1269486 of GATA3 was associated with AR and sensitivity to aeroallergens in our population. Because of the significance of this gene in AR, studying the association between GATA3 polymorphisms and AR is recommended for other populations.
Authors: Maritta Pykäläinen; Riikka Kinos; Sanna Valkonen; Pia Rydman; Maritta Kilpeläinen; Lauri A Laitinen; Jussi Karjalainen; Markku Nieminen; Mikko Hurme; Juha Kere; Tarja Laitinen; Riitta Lahesmaa Journal: J Allergy Clin Immunol Date: 2005-01 Impact factor: 10.793
Authors: Y Yokouchi; M Shibasaki; E Noguchi; J Nakayama; T Ohtsuki; M Kamioka; K Yamakawa-Kobayashi; S Ito; K Takeda; K Ichikawa; Y Nukaga; A Matsui; H Hamaguchi; T Arinami Journal: Genes Immun Date: 2002-02 Impact factor: 2.676
Authors: E Noguchi; Y Yokouchi; M Shibasaki; M Kamioka; K Yamakawa-Kobayashi; A Matsui; T Arinami Journal: Genes Immun Date: 2001-11 Impact factor: 2.676