Literature DB >> 2836707

Cloning and characterization of the gene, speC, for pyrogenic exotoxin type C from Streptococcus pyogenes.

S C Goshorn1, G A Bohach, P M Schlievert.   

Abstract

The structural gene of streptococcal pyrogenic exotoxin type C (SPE C) was cloned from the chromosome of Streptococcus pyogenes strain T18P into Escherichia coli using pBR328 as the vector plasmid. Subcloning enabled the localization of the gene (speC) to a 1.7 kb fragment. Partially purified E. coli-derived SPE C and purified streptococcal-derived SPE C, were shown to have the same molecular weight (23,800) and biological activities. A DNA probe, prepared from cloned speC, cross-hybridized with the structural genes of SPE A and SPE B indicating relatedness at the nucleotide level. The speC-derived probe also hybridized to a fragment of CS112 bacteriophage DNA containing the phage attachment site.

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Year:  1988        PMID： 2836707     DOI： 10.1007/BF00322445

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  34 in total

1.  ENHANCEMENT OF BACTERIAL ENDOTOXIN LETHALITY BY STAPHYLOCOCCAL ENTEROTOXIN.

Authors:  H SUGIYAMA; E M MCKISSIC; M S BERGDOLL; B HELLER
Journal:  J Infect Dis       Date:  1964-04       Impact factor: 5.226

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage.

Authors:  B N Kreiswirth; S Löfdahl; M J Betley; M O'Reilly; P M Schlievert; M S Bergdoll; R P Novick
Journal:  Nature       Date:  1983 Oct 20-26       Impact factor: 49.962

5.  A physical map of the group A streptococcal pyrogenic exotoxin bacteriophage T12 genome.

Authors:  L P Johnson; P M Schlievert
Journal:  Mol Gen Genet       Date:  1983

6.  Recovery of DNA from gels.

Authors:  H O Smith
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  A gas-liquid solid phase peptide and protein sequenator.

Authors:  R M Hewick; M W Hunkapiller; L E Hood; W J Dreyer
Journal:  J Biol Chem       Date:  1981-08-10       Impact factor: 5.157

8.  A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.

Authors:  M S Blake; K H Johnston; G J Russell-Jones; E C Gotschlich
Journal:  Anal Biochem       Date:  1984-01       Impact factor: 3.365

9.  Toxic-shock-syndrome toxin 1-induced proliferation of lymphocytes: comparison of the mitogenic response of human, murine, and rabbit lymphocytes.

Authors:  N J Poindexter; P M Schlievert
Journal:  J Infect Dis       Date:  1985-01       Impact factor: 5.226

10.  A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.

Authors:  R M Dale; B A McClure; J P Houchins
Journal:  Plasmid       Date:  1985-01       Impact factor: 3.466
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  13 in total

1.  Production of pyrogenic exotoxins in group A streptococci isolated from patients in Zagreb, Croatia.

Authors:  J Begovac; B Gmajnicki; P M Schlievert; D R Johnson; E L Kaplan
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-06       Impact factor: 3.267

2.  Streptococcus pyogenes causing toxic-shock-like syndrome and other invasive diseases: clonal diversity and pyrogenic exotoxin expression.

Authors:  J M Musser; A R Hauser; M H Kim; P M Schlievert; K Nelson; R K Selander
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-01       Impact factor: 11.205

3.  Streptococcal pyrogenic exotoxin F (SpeF) causes permeabilization of lung blood vessels.

Authors:  M Matsumoto; N Ishikawa; M Saito; K Shibayama; T Horii; K Sato; M Ohta
Journal:  Infect Immun       Date:  1999-09       Impact factor: 3.441

4.  Molecular epidemiologic analysis of the type A streptococcal exotoxin (erythrogenic toxin) gene (speA) in clinical Streptococcus pyogenes strains.

Authors:  C E Yu; J J Ferretti
Journal:  Infect Immun       Date:  1989-12       Impact factor: 3.441

5.  Nucleotide sequence of streptococcal pyrogenic exotoxin type C.

Authors:  S C Goshorn; P M Schlievert
Journal:  Infect Immun       Date:  1988-09       Impact factor: 3.441

6.  Group A streptococcal pyrogenic exotoxin (scarlet fever toxin) type A and blastogen A are the same protein.

Authors:  P M Schlievert; E D Gray
Journal:  Infect Immun       Date:  1989-06       Impact factor: 3.441

7.  Biological and immunological properties of the carboxyl terminus of staphylococcal enterotoxin C1.

Authors:  G A Bohach; J P Handley; P M Schlievert
Journal:  Infect Immun       Date:  1989-01       Impact factor: 3.441

8.  Molecular analysis of pyrogenic exotoxins from Streptococcus pyogenes isolates associated with toxic shock-like syndrome.

Authors:  A R Hauser; D L Stevens; E L Kaplan; P M Schlievert
Journal:  J Clin Microbiol       Date:  1991-08       Impact factor: 5.948

9.  Bacteriophage association of streptococcal pyrogenic exotoxin type C.

Authors:  S C Goshorn; P M Schlievert
Journal:  J Bacteriol       Date:  1989-06       Impact factor: 3.490

10.  Site-specific manifestations of invasive group a streptococcal disease: type distribution and corresponding patterns of virulence determinants.

Authors:  Bart J M Vlaminckx; Ellen M Mascini; Joop Schellekens; Leo M Schouls; Armand Paauw; Ad C Fluit; Rodger Novak; Jan Verhoef; Franz Josef Schmitz
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948
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