Mei Huang1, Robert G Hill2, Simon C F Rawlinson3. 1. Institute of Dentistry, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, UK; Institute of Dentistry, Dental Physical Sciences Unit, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, UK. Electronic address: mei.huang@qmul.ac.uk. 2. Institute of Dentistry, Dental Physical Sciences Unit, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, UK. 3. Institute of Dentistry, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, UK.
Abstract
OBJECTIVE: A promising strategy in regenerative endodontics is the combination of human dental pulp stem cells (hDPSCs) with an appropriate biomaterial substrate. The effects of zinc and zinc containing bioactive glasses (ZnBGs) on hDPSCs have been characterized in this study. METHODS: ZnBGs were designed and produced. Then the odontogenic differentiation and mineralization potential of hDPSCs upon ZnBGs treatment were investigated. RESULTS: Free Zn ions (0-5ppm) enhanced proliferation and alkaline phosphatase (ALP) activity of hDPSCs. Further, ZnBGs conditioned medium (ZnBG-CM) increased the production and secretion of odontogenic markers: dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1). In addition, we identified that mRNA expression of the osteogenic markers RUNX2, OCN, BSP, BMP-2, MEPE and ON was increased following treatment with ZnBG-CM. Long term treatment with ZnBG-CM increases the formation rate of mineralized nodules (similar to hydroxyapatite, Ca:P=1.6), as confirmed by scanning electron microscopy combined with energy dispersive X-ray spectroscopy (SEM-EDX). Lastly, the administration of ZnBG-CM induces VEGF expression. SIGNIFICANCE: These findings implicate that ZnBG would be beneficial in regenerative endodontics and could influence the way present Zn containing clinical products are used.
OBJECTIVE: A promising strategy in regenerative endodontics is the combination of human dental pulp stem cells (hDPSCs) with an appropriate biomaterial substrate. The effects of zinc and zinc containing bioactive glasses (ZnBGs) on hDPSCs have been characterized in this study. METHODS:ZnBGs were designed and produced. Then the odontogenic differentiation and mineralization potential of hDPSCs upon ZnBGs treatment were investigated. RESULTS: Free Zn ions (0-5ppm) enhanced proliferation and alkaline phosphatase (ALP) activity of hDPSCs. Further, ZnBGs conditioned medium (ZnBG-CM) increased the production and secretion of odontogenic markers: dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1). In addition, we identified that mRNA expression of the osteogenic markers RUNX2, OCN, BSP, BMP-2, MEPE and ON was increased following treatment with ZnBG-CM. Long term treatment with ZnBG-CM increases the formation rate of mineralized nodules (similar to hydroxyapatite, Ca:P=1.6), as confirmed by scanning electron microscopy combined with energy dispersive X-ray spectroscopy (SEM-EDX). Lastly, the administration of ZnBG-CM induces VEGF expression. SIGNIFICANCE: These findings implicate that ZnBG would be beneficial in regenerative endodontics and could influence the way present Zn containing clinical products are used.
Authors: C Pushpalatha; Jithya Suresh; V S Gayathri; S V Sowmya; Dominic Augustine; Ahmed Alamoudi; Bassam Zidane; Nassreen Hassan Mohammad Albar; Shankargouda Patil Journal: Front Bioeng Biotechnol Date: 2022-05-19